HPLC法测定白山片中苦参碱的含量

Determination of Matrine in Baishan Tablets by HPLC

目的：建立以高效液相色谱法测定白山片中苦参碱含量的方法。方法：色谱柱为氨基键合柱（200mm×4．6mm，10μm），流动相为乙腈-0．5％磷酸溶液乙醇（100：8：10），检测波长为220nm，流速为1．0mL·min，枉温为30℃。结果：苦参碱工壹样量在0．27～8．66μg范围内与峰面积积分值呈良好的线性关系（r=0．9998）；平均加样回收率为99．52％，RSD=0．91％（n=6）。结论：本方法简便、准确、重现性好，可用于白山片的质量控制。

OBJECTIVE： To establish an HPLC method for the determination of Matrine in Baishan tablets. METHODS： The analytical column was Aminopropyl （200 mm×4.6 mm, 10 μm） with mobile phase consisted of acetonitrile 0.5% phosphate solution - ethanol（100 ： 8 ： 10） at a flow rate of 1.0 mL · min^-1. The column temperature was set at 30 ℃ and the detection wavelength was set at 220 nm. RESULTS： The linear range of Matrine was 0.27- 8.66 μg（r = 0.999 8） and the average recovery of Matrine was 99.52 % （RSD = 0.91%, n = 6） . CONCLUSION： The method is simple, accurate and reproducible, and it is applicable for the quality control of Baishan tablets.