HPLC法测定妇安口服液中阿魏酸的含量

Determination of Ferulic Acid in Fuan Oral Liquid by HPLC

目的：建立以高效液相色谱法测定妇安口服液中阿魏酸含量的方法。方法：色谱柱为Hypersil BDS C18（250mm×4．6mm，5μm），流动相为甲醇-0．01mol·L^-1磷酸二氢钾溶液（磷酸调pH3．7），梯度洗脱，检测波长为323nm，流速为1mL·min^-1.结果：阿魏酸的检测浓度在8～40μg·mL^-1范围内与峰面积积分值呈良好的线性关系（r=0．9994）；平均回收率为99．03％，RSD=0．63％（n=6）。结论：本方法简便、快捷、结果准确，可用于妇安口服液的质量控制。

OBJECTIVE： To establish an HPLC method for the determination of Ferulic acid in Fuan oral liquid, METHODS： The sample was separated on Hypersil BDS C18（250 mm × 4.6 mm, 5μm） column. The mobile phase consisted of methanol - potassium dihydrogen phosphate （with pH adjusted to 3.7 by phosphoric acid） in a mode of gradient elution. The UV detective wavelength was set at 323 nm. The flow rate was set at 1 mL · min ^-1.RESULTS： The linear range of Ferulic acid was 8-40μg · mL^ -1（ r = 0.999 4） ,The average recovery was 99.03% （RSD = 0.63%, n = 6） . CONCLUSION： This method is simple, rapid and accurate, and it can be used for quality control of Fuan oral liquid.