全血中维生素E和辅酶Q10含量的高效液相色谱法测定

Determination of Vitamin E and CoQ_(10) in Whole Blood by High Perfomance Liquid Chromatography

建立了一种同时测定全血中维生素E和辅酶Q10的高效液相色谱法。样品经乙醇沉淀蛋白，以正己烷萃取，氮气吹干后，流动相溶解，以甲醇-乙醇（体积比20：80）为流动相，在C18柱（5μm，250mm×4．6mm i．d．）上进行色谱测定。维生素E和辅酶Q10的线性范围皆为1．0～50．0mg／L，检出限分别为0．21、0．15mg／L，相对标准偏差分别为5．2％、5．9％；全血样品的维生素E和辅酶Q10加标回收率分别为97％～115％和90％～112％。

A method for the determination of vitamin E and CoQ10 in whole blood by HPLC was developed. After precipitation of proteins with anhydrous ethanol, vitamin E and CoQ10 in blood were extracted with hexane and then determined with HPLC. A C18 column（ Phenomenex, 5μm, 250 mm × 460 mm） and the mobile phase（20 ： 80 methanol -ethanol by volume） were used throughout the experiment. The detection wavelength was 292 nm for 8 min, then changed to 275 nm. The linear ranges were 1.0 -50. 0 mg/L, the detection limits were 0.21 mg/L and 0. 15 mg/L, the relative standard deviation（RSD） for vitamin E and CoQ10 were 5.2% and 5.9% , the recoveries were 97% - 115% and 90% -112% for vitamin E and CoQ10, respectively.