三棱丸抑制大鼠异位子宫内膜芳香化酶及环氧合酶-2表达的研究

Inhibitory effects of Sanlengwan on aromatase and cyclooxygenase-2 in rats of endometriosis

目的:探讨三棱丸(SLW)抑制内异症大鼠异位子宫内膜局部雌激素的生成及其作用机制。方法:采用子宫内膜自体移植法建立大鼠子宫内膜异位症模型,将40只造模造模成功大鼠随机分为5组(n=8):模型对照组,SLW高、中、低剂量组和阿那曲唑组,另设正常对照组。分别灌胃给药4周后,免疫组化和western blot方法检测异位内膜组织中细胞色素P450芳香化酶(P450 arom)、环氧合酶-2(COX-2)蛋白的表达,发光免疫分析法及放射免疫分析法分别检测异位内膜组织雌二醇(E2)及前列腺素E2(PGE2)的含量。结果:SLW各剂量组能有效抑制异位内膜组织中P450 arom的表达,降低其表达产物雌二醇的生成水平,与模型对照组比较,差异显著(P<0.01);SLW高剂量组能降低异位子宫内膜环氧合酶-2的表达,其产物前列腺素E2的水平也明显低于模型对照组(P<0.05)。结论:SLW具有良好的抗EMS雌激素生成作用,其作用与抑制P450 arom表达,阻断雌激素生成的正反馈环有关。

Objective： To explore the effect mechanism of Sanlengwan （SLW） on estrogen production in ectopic endometrium of rats. Method. The rat model of endometriosis was established by surgical implant of endometrial tissue which belong to its body. Forty EMS model rats were randomly divided into five groups （n = 8） - model control group, three different concentration SLW groups and anastrozole group. Meanwhile, eight normal rats were used as the normal control group. All the rats were treated for 4 weeks re- spectively, the changes of the P450 arom and cyclooxygenase-2 protein were measured by immunohistochemical test and western blot re- spectively before and after treatment of SLW, and the level of secretion of estrodiol and prostaglandin E2 was also measured by ECLIA and RIA. Result： SLW can reduce the expression of P450 arom protein ,and the levels of estradiol after treatment of SLW were significantly lower than that of the model group in ectopic endometrial tissue （ P 〈 0. 05 ） ; The high dose group of SLW can inhibit the expression of cyclooxygenase-2 protein and also reduce the production of prostaglandin E2 （ P 〈 0. 05 ）. Conclusion： SLW can reduce the pro- duction of estradiol in the ectopic endometrial tissue of rats, and its mechanism might be associated with inhibiting the expression of P450 arom and interruption the positive feedback loop of estradiol production.