八味锡类散对大鼠溃疡性结肠炎CD3，CD4，CD8T淋巴细胞的影响

Effect of Bawei Xilei powder on CD3,CD4,CD8 T-lymphocytes of rats with ulcerative colitis

目的:观察八味锡类散对大鼠溃疡性结肠炎外周血及结肠黏膜T淋巴细胞亚群的影响。方法:将60只SD大鼠随机分为6组(即正常组、模型组、八味锡类散低剂量组、八味锡类散中剂量组、八味锡类散高剂量组、柳氮磺吡啶对照组)。用兔黏膜免疫法造模后,灌肠给药18 d。运用流式细胞术及免疫组化染色法观察外周血及结肠黏膜的T淋巴细胞亚群的表达。结果:在外周血方面,与正常组比较,模型组CD4 T淋巴细胞升高、CD8 T淋巴细胞降低、CD4/CD8升高均有极显著性差异(P<0.01)。与模型组比较,八味锡类散对CD8 T淋巴细胞有升高趋势,但仅八味锡类散高剂量组有显著性差异(P<0.05)。对CD4/CD8治疗各组均有降低趋势,除八味锡类散低剂量组外,其他治疗组均有显著性差异(P<0.05);在结肠黏膜方面,与正常组比较,模型组可降低CD3,CD4 T淋巴细胞的表达、升高CD8 T淋巴细胞水平且均有显著差异(P<0.05或P<0.01),与模型组比较,八味锡类散各组及西药对照组均可降低CD8 T淋巴细胞水平并有显著差异(P<0.05或P<0.01)。结论:八味锡类散可以调节T淋巴细胞亚群之间的平衡,从而减轻炎症损伤,有利于溃疡的修复和组织再生。

Objective： To observe the effect of Bawei Xilei powder on CD3, CD4, CD8 T-lymphocytes in peripheral blood and colonic mucosa of rat with ulcerative colitis. Method： Sixty SD rats were randomly divided into 6 groups, normal group, model group, low, middle and high dosage Bawei Xilei powder group, Sulfasalazine group. Ulcerative colitis was induced by immunization with rabbit colonic mucous emulsified with completely Freund＇s adjuvant in all rats. Rats in low, middle and high dosage Bawei Xilei powder group were administered with 0. 05, 0. 1, 0. 2 mg Bawei Xilei powder for 18 days by enema respectively. While rats in Sulfasalazine group were enema administered with 100 mg Sulfasalazine, and the rats in other group were administered with equal volume of saline enema as control. We analyzed expression of CD3, CD4, CD8 T-lymphocytes in peripheral blood by flow cytometry and in colonic mu- cous by immunohistochemistry. Result： In peripheral blood, compared with normal group, in model group, the increased of CD4 T- lymphocytes and CD4/CD8 ratio, the reduced of CD8 T-lymphocytes, these results were significant discrepancy （ P 〈 0. 01 ）. Com- pared with model group, after treatment with Bawei Xilei powder, CD8 T-lymphocytes increased, but only high dosage Bawei Xilei powder group had discrepancy （ P 〈 0. 05 ）. But low dosage Bawei Xilei powder group, other treatment groups rats showed CD4/CD8 ratio were reduced significantly （ P 〈 0. 05）. In colonic mucous, compared with normal group, in model group, Rats showed that expression of CD3, CD4 T-lymphocytes reduced and CD8 T-lymphocytes increased obviously （ P 〈 0. 05, P 〈 0. 01 ）. Compared with model group, expression of CD8 T-lymphoeytes reduced significantly in all treatment groups （P 〈0. 05, P 〈0. 01 ）. Conclusion： Ba- wei Xilei powder may regulate their balance between T-lymphocytes subgroup, consequently relieve inflammatory injury in favor of ulcer reparation and tissue regeneration.