风轮菜乙醇提取物的降血糖作用及其机制研究

Studies on anti-hyperglycemic effect and its mechanism of Clinopodium chinense

目的:研究风轮菜醇提物(EJCT)的降血糖作用及其机制。方法:观察EJCT 150,300,600 mg.kg-1对正常小鼠空腹血糖(FSG)的影响;对肾上腺素性高血糖小鼠血糖及肝糖原、四氧嘧啶糖尿病小鼠血糖及胰岛损伤程度的影响。观察EJCT对Fe2+/Cys激发的小鼠肝匀浆过氧化产物丙二醛(MDA)的影响。结果:EJCT对正常小鼠血糖无明显影响。但是,EJCT 300,600 mg.kg-1可显著降低肾上腺素所致的小鼠血糖升高,并使低下的肝糖原回升。EJCT 150,300,600 mg.kg-1明显降低四氧嘧啶糖尿病小鼠的血糖及减轻四氧嘧啶对胰岛细胞的损伤。另外,EJCT 30,60,90,120 mg.L-1能明显降低Fe2+/Cys激发的小鼠肝匀浆中MDA的含量。结论:EJCT具有显著的降血糖作用,其作用机制可能与增加肝糖原合成、减少肝糖原分解、提高机体抗脂质过氧化能力,从而减轻胰岛细胞的损伤有关。

Objective： To investigate the anti-hyperglycemic effect and its mechanism of ethanol extraction from Calamintha chinensis （EJCT）. Method： Fasting serum glucose （FSG） in normal mice was determined after oral administration of EJCT. Effects of EJCT on hyperglycemia mice induced by adrenaline were investigated by observing the contents of FSG and liver glucogen. Effect of EJCT on the diabetic mice induced by alloxan was investigated by observing the contents of FSG and the injured degree of pancreatic islet. The antilipid-peroxidation of EJCT on liver homogenate was measured by determination of malondiadehyde （MDA） induced by Fe2＋/Cys. Result： EJCT showed no obvious effect on FSG in normal mice. However, EJCT 300,600 mg· kg-1 could remarkably de- crease the contents of FSG and increase liver glucogen in hyperglycemia mice induced by adrenaline. In diabetic mice induced by alloxan, EJCT 150,300,600 mg· kg-1 could remarkably decrease the contents of FSG. The damage of pancreatic islet induced by alloxan was also significantly attenuated by EJCT. Furthermore, EJCT 30, 60, 90, 120 mg ·L-1 inhibited lipid peroxidation initiated by Fe2＋/Cys in liver homogenate. Conclusion： These results suggest that EJCT can significantly attenuate hyperglycemia in diabetic mice, which is probably due to decreasing the decomposition of liver glucogen, increasing the synthesis of liver glucogen, antioxidation and amelioration of damaged pancreatic islet.