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生物素标记的核酸分子杂交 被引量：2

HYBRIDIZATION WITH BIOTINYLATED PROBE
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摘要 用生物素代替同位素标记探针技术、NaAc－NaOH溶液代替Tris－Nacl中和液、脱脂奶粉代替Denhardt溶液和小牛胸腺DNA进行分子杂交。结果表明：经改进后的核酸分子杂交方法具有简便、安全、稳定、成本低的优点。 Researches were made on replacing isotope by biotin; Tris-NaCl solution by NaAcNaOH solution;Denhardt's solution and calf thymus DNA by de-fatted milk powder in hybridization. The results indicated that the improved method possesses the advantages of simplicity, safety, stability and cheapness.
作者 徐宏斌
出处 《皖南医学院学报》 CAS 1997年第4期430-432,共3页 Journal of Wannan Medical College
关键词 生物素 分子杂交 核酸 标记 DNA biotin probe Southern blot hybridization
分类号 Q523.03 [生物学—生物化学]
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同被引文献36

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  • 6De-Vries BB, Knight SJ, Homfray T, Smithson S-F et al. Submicroscopic subtelomeric lqter deletions: a recognisable phenotype [J]? J Med Genet, 2001, 38(3): 175-178.
  • 7Orsetti B, Lefort G, Boulot P, et al. Fetal cells in maternal blood: the use of primed in situ (PR1NS) labelling technique for fetal cell detection and sex assessment [J]. Prenat Diagn, 1998, 18(10):1014-1022.
  • 8Thiel CT, Kraus C, Rauch A, et al. A new quantitative PCR multiplex assay for rapid analysis of chromosome 17p11.2-12 duplications and deletions leading to HMSN/HNPP [J]. Eur J Hum Genet, 2003, 11(2): 170-178.
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  • 10Drobyshev A, Mologina N, Shik V, et al. Sequence analysis by hybridization with oligonucleotide microchip: identification of beta-thalassemia mutations [J]. Gene, 1997, 188(1): 45-52.

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皖南医学院学报
1997年 第4期

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