摘要
目的 考察基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）用于检测天然多糖相对分子质量的可行性及条件。方法 以2，5-二羟基苯甲酸（DHB）为基质对精制后的天然白芨多糖（未分级）相对分子质量进行测定。条件采用DHB-白芨多糖为1．5：1，室温液滴自然干燥法；线性正离子谱模式，激光强度：1500～2500单位。结果 白芨多糖相对分子质量分布在6×10^4～3×10^5，其分散性较大。结论 采用DHB与白芨多糖样品比例为1．5：1，直接液滴干燥法效果好，能获得较好的图谱效果。
Objective To investigate the relative molecular weight of polysaccharides in Bletilla striata by matrix-assisted laser desorption ionizatior/time-of-flight mass （MALDI-TOF-MS）. Methods 2, 5- Dihydroxybenzoic acid （DHB） as a matrix was used. By drop drying at room temperature the proportion of the matrix and polysaccharide sample was 1.5 ： 1. Baseline mode and laser intensity at 1 500-2 500 units were carried out. Results The relative molecular weights of polysaccharides in B. striata were 6×10^4-3×10^5, whose distribution rang was wide. Conclusion The relative molecular weights of polysaccharides in B. striata are obtained by MALDI-TOF-MS technique in the proportion of DHB-polysaccharides in B. striata 1.5 ： 1, which is valuable for the preparation and good quality by the direct drop drying method.
出处
《中草药》
CAS
CSCD
北大核心
2008年第6期817-819,共3页
Chinese Traditional and Herbal Drugs
关键词
白芨多糖
基质辅助激光解吸电离飞行时间质谱
基质
相对分子质量
polysaccharides in Bletilla striata （Thunb.） Reichb. f.
matrix-assisted laser desorption ionizatior/time-of-flight mass （MALDI-TOF-MS）
matrix
relative molecular weight
