温血诱导及终未再灌注对未成熟心肌保护的实验研究

Experimental studies of immature myocaudial protection with warm induction and reperfusion

为研究温血停搏液（谷氨酸加天门冬氨酸强化）诱导停跳和主动脉开放前再灌注的未成熟心肌保护的效果。用12只新西兰幼免（3～4周），体重450～550g，随机分成2组：冷血停搏液（谷氨酸加天门冬氨酸强化）组（Ⅰ组，n＝6）；温血停搏浪诱导及再灌注血组（Ⅱ组，n＝6）。结果：1．心功能；CO±dp0／dt恢复百分率Ⅱ组明显优于Ⅰ组（P＜0．01）；LVSP恢复百分率Ⅱ组优于Ⅰ组（P＜0．05）。2．LDH和CK漏出量（U／l）Ⅱ组明显优于Ⅰ组（P＜0．01）；3。心肌含水量（％）Ⅱ组优于Ⅰ组（P＜0．01）；4．心肌结构保护Ⅱ组明显优于Ⅰ组。本实验提示：温血诱导停跳与主动脉开放前温血再灌注对于未成熟心肌有良好的心肌保护效果。

The objective of this study is to examine the myocardial protection effect of warm induction be foreclamping and reperfusion be fore unclamping blood cardioplegia in immature heart. Twelve Newzealand rabbits(aged 3 to4 weeks) are divided randomly into two groups:Group I, aspartate (20mmol/1 ) and glutamate (20mm0l/1 ) -enrichedblood cardioplegia (n= 6):Group I,warm induction and reper fusion (n= 6). Results 1. percent recovery of CO and ±dp/dt:group I versus Group I (P<0. 01)；percent recovery of LVSP: Group Ⅱversus Group I (P<0. 01). 2. LDHand CK realease (u/l): Group Ⅱversus Group I (P<0. 01 ). 3. myocardial water content (% ):group, versus GroupI (P<0. 05), Group I versus Group I (P<0. 01 ) 4. protection of myocardial structure was more excellent in GroupI than Group I under electrical microgrphy- Conclusion was achieved from the results that warm induction be foreclamping and reperfusion before unclamoing afford excellent myocardial protection and have a good future for the surgi-cal repairing of complex c0ngenital heart anomalies by direct vision.