摘要
目的研究离体条件下射干试管根茎诱导的最适培养基。方法利用植物组织培养技术,研究了碳源种类和质量浓度,NAA质量浓度和活性炭对射干试管根茎诱导的影响。结果射干试管根茎诱导的最佳培养基是MS+6-BA2.0mg/L+NAA0.5mg/L+白糖6%,培养基中不宜添加活性炭。试管根茎接入培养基MS+BA2.0mg/L+白糖3%上,发芽率达61.03%。结论糖质量浓度是影响射干试管根茎诱导的主要因素。
Objective To optimize the medium for rhizomatous induction of Belarncanda chinensis in vitro. Methods By plant tissue culture technology, the effects of various carbon source, NAA, and active carbon at different concentration on the rhizomatous formation of B. chinensis in vitro were studied. Results MS＋6-BA 2.0 mg/L＋NAA 0. 5 mg/L＋6% white sugar was the optimal medium for the rhiz- omatous formation of B. chinensis in vitro. Active carbon should not be added to the medium. The germination rate of rhizomatous in vitro was 61.03% on the MS ＋ BA 2.0 mg/L＋3% white sugar. Conclusion Sugar concentration is the main factor of the influence on the rhizomatous formation of B. chinensis in vitro.
出处
《中草药》
CAS
CSCD
北大核心
2008年第6期910-913,共4页
Chinese Traditional and Herbal Drugs
关键词
射干
根茎
诱导
Belamcanda chinensis （L.） DC.
rhizome
induction
