基于指标成分和HPLC指纹图谱检测的决明子化学品质评价研究

Quality evaluation of Semen Cassiae by both indicated component determination and HPLC fingerprint

目的 客观评价决明子内在质量差异。方法 采用HPLC方法测定大黄酚的量；采用梯度洗脱法建立HPLC指纹图谱；指纹图谱色谱条件：C18柱（250mm×4．6mm，5μm），流动相为乙腈和1％醋酸，检测波长为254nm，柱温为室温，体积流量为1mL／min；利用中药色谱指纹图谱相似度评价软件对不同产地决明子指纹图谱进行相似度分析；以大黄酚的量和指纹图谱相似度为指标，运用SAS软件进行聚类分析。结果 建立的指纹图谱精密度、重现性、稳定性好，不同产地决明子的大黄酚的量及化学指纹图谱不尽相同，化学成分差异与产地相关，大黄酚质量分数为0．037％～0．170％，指纹图谱相似度范围为0．864～0．962。结论 综合指标成分定量测定及化学指纹图谱分析方法，能较全面地表征不同产地决明子的内在成分差异，可为决明子内在质量控制提供参考。

Objective To evaluate the quality of Semen Cassiae from different habitats objectively. Methods To determine the content of chryso-phanic according to ChP and establish HPLC fingerprints with the gradient elution solvent composed of acetonitrile and 1% HAC. A C18column （250 mm×4.6 mm, 5μm） was used, flow rate： 1 mL/min, detecting wavelength. 254 nm, and the column temperature, room temperature. The clustering analysis was carried out by SAS software according to the content of chrysophanic and similarity of HPLC fingerprints obtained by the software of similarity analysis. Results The established HPLC fingerprint has desirable precision, reproducibility, and stability. The content of chrysophanic and HPLC fingerprints of Semen Cassiae from various habitats are different, which differs from the habitats. The content range of chrysophanic in Semen Cassiae is 0. 037%-0. 170% and the similarity is 0. 864-0. 962. Conclusion The method indicates the difference of the chemical component in Semen Cassiae from various habitats and can be used as a quality control method for Semen Cassiae.