活血化瘀法对慢性萎缩性胃炎癌前病变大鼠早期细胞凋亡的影响

Analysis of early apoptosis of precancerous lesion of rats with chronic atrophic gastritis treated by the therapy of activating blood and resolving blood stasis

目的研究活血化瘀法、理气活血法和益气活血法对慢性萎缩性胃炎(Chronic Atrophic Gastritis,CAG)癌前病变大鼠早期细胞凋亡的影响。方法采用幽门弹簧插入配合高盐热糊灌胃法复制CAG癌前病变模型,用双标记流式细胞术检测癌前病变大鼠早期细胞凋亡。结果益气活血法、活血化瘀法和理气活血法干预12周后,自然恢复组大鼠早期凋亡率明显高于空白对照组(P<0.01),各干预组早期细胞凋亡率均有降低,其中活血化瘀组、理气活血组与自然恢复组比较有显著性差异(P<0.01或P<0.05),益气活血组显示出降低早期细胞凋亡的趋势,但与自然恢复组比较未见明显差异(P=0.057)。结论益气活血法、活血化瘀法和理气活血法对CAG癌前病变大鼠早期细胞凋亡均有抑制作用,这可能是益气活血法、活血化瘀法和理气活血法改善或逆转大鼠CAG癌前病变的作用机理之一。

Objective To study the early apoptosis of precancerous lesion of chronic atrophic gastritis（CAG） rats treated by blood activating and blood stasis resolving therapy,qi moving and blood activating therapy and qi replenishing and blood activating therapy.Methods The CAG rat model was replicated by methods of spring insertion in pylorus and oral administration with high salt hot paste.The early apoptosis of precancerous lesion of CAG rats treated by three therapise was detected using Annexin V FITC/PI double labeled flow cytometry.Results The ratio of the early apoptosis of the model control group increased significantly when compared with the blank control group（P〈0.01）.The ratio of the early apoptosis of the groups treated by the three therapies decreased when compared with the model contral group,with significant differences in the blood activating and blood stasis resolving therapy group and the qi moving and blood activating therapy group.（P〈0.01,P〈0.05）.Conclusions Restraining the apoptosis may be one of the mechanisms for blood activating and blood stasis resolving therapy,qi moving and blood activating therapy and qi replenishing and blood activating therapy to improve and inverse the precancerous lesion of CAG rats.