脑热清对内毒素性发热家兔清热化瘀作用的研究

Heat-clearing and stasis-resolving effect of Naoreqing on rabbits with endotoxin-induced fever

目的探讨脑热清口服液对内毒素性发热家兔清热化瘀的作用。方法复制大肠杆菌内毒素性发热家兔模型,用数字温度计测量家兔的直肠温度,采用放免法测定血浆血栓素B2（TXB2）、6-酮-前列腺素F1α（6-keto-PGF1α）及血清肿瘤坏死因子（TNFα）的含量;用发色底物法测定血浆组织纤溶酶原激活物（t-PA）和组织纤溶酶原激活物抑制剂（PAI）的活性。同时取心、肝、肺、肾组织,观察形态改变。结果脑热清对内毒素性发热家兔有显著的解热效应（P（0.01）;脑热清可明显抑制发热家兔TNFα、TXB2的含量（P（0.05~0.01）,降低PAI的活性（P（0.01）,同时可提高6-keto-PGF1α的含量和t-PA的活性（P（0.01）;光镜显示,脑热清可减轻发热家兔心、肝、肺、肾血管扩张充血、瘀血的病理形态改变。结论脑热清可能通过抑制致热性细胞因子释放和调节血管内皮细胞分泌功能达到清热化瘀作用。

Objective To observe the heat-clearing and stasis-resolving effect of Naoreqing on rabbits with endotoxin-induced fever.Methods The endotoxin-induced fever model of rabbit was established.The digital thermometer was used to measure rectal temperature.The Thromboxane B2（TXB2） in plasma,6-Keto-Prostaglandin F1α（6-keto-PGF1α） and tumor necrosis factor-α（TNFα） in serum were determined by radioimmunoassay.The activity of tissue-type plasminogen activator（t-PA） and plasminogen activator inhibitor（PAI）in plasma were measured by the assay of chromogenic substrate.At the same time,the morphologic changes of the tissues of heart,liver,lung and kidney were observed.Results NRQ had obvious antipyretic effect on the rabbits with endotoxin-induced fever（P﹤0.01）;NRQ reduced TNFα content in serum and TXB2 content in plasma（P﹤0.05～0.01）,and decreased the activity of PAI in plasma（P﹤0.01）. NRQ enhanced the activity of t-PA in plasma,and increased the 6-keto-PGF1α content（P﹤0.01）;Showed by light microscope,NRQ reduced pathological changes caused by hyperemia and congestion of blood vessel in heart,liver,lung and kidney of febrile rabbits.Conclusion NRQ has the effect of clearing heat and resolving stasis.The mechanism is probably that NRQ can inhibit the secretion of cytokine TNFα and adjust the secrete function of vascular endothelial cells.