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脂质体介导pcDNA3．1-HGF瞬时转染小鼠MSCs及转染效果检测 被引量：1

TRANSIENT TRANSFECTION OF PCDNA3.1-HGF IN MOUSE MESENCHYMAL STEM CELLS
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摘要 肝细胞生长因子(HGF)对各种组织都具有促分裂、促迁移、促细胞形态发生的作用,尤其是肾脏、肺和肠道.间充质干细胞(MSC)是一种具有多向分化潜能的干细胞,同时还具有相对的定位归巢以及免疫调节作用,由于MSC具有较低的免疫原性,可作为良好的基因载体.为联合应用HGF和MSC的作用,本实验在分离培养小鼠MSC并鉴定后;应用脂质体介导法将pcDNA3.1-HGF瞬时转染MSC;采用半定量RT-PCR和ELISA法分别鉴定HGF的表达情况.结果表明:转染试剂对MSC的活性无影响,转染细胞形态及定向分化特性未发生改变;HGF在转染后48 h达到最高表达量,随时间推移逐渐减低,在10 d时仍略高于对照.脂质体介导pcDNA3.1-HGF瞬时转染小鼠MSC后,HGF在转染后10 d内升高表达. Hepatocyte growth factor （HGF） has been applied in tissue repair. In this study, bone marrow-derived mesenchymal stem cells （MSC） were isolated, expanded, characterized, and transfected with plasmid pcDNA3. 1-HGF. Reversed transcription polymerase chain reaction （RT-PCR） and ELISA analysis of mRNA and protein showed clear HGF expression at high level, which could he maintained for about 10 days. It is therefore concluded that transient HGF gene transfection in MSC could be done with the liposome method.
作者 黄雅静 孙万军 孙琪云 王冬梅 艾辉胜
机构地区 第二炮兵总医院 军事医学科学院附属医院
出处 《北京师范大学学报（自然科学版）》 CAS CSCD 北大核心 2008年第3期259-262,共4页 Journal of Beijing Normal University(Natural Science)
关键词 间充质干细胞 肝细胞生长因子 脂质体 mesenchymal stem cells hepatocyte growth factor liposome
分类号 Q781 [生物学—分子生物学] S827 [农业科学—畜牧学]
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参考文献12

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同被引文献7

  • 1金泰廙.职业卫牛与职业医学.北京:人民卫生出版社,2003:249.
  • 2Brown T. Silica exposure, smoking, silicosis and lung cancer-com- plex interactions. Occup Med (Lond), 2009, 59: 89-95.
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  • 4Ortiz LA, Gambelli F, McBride C, et al. Mesenchymal stem cell en- graftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci U S A, 2003,100:8407-8411.
  • 5Marchand-Adam S, Marchal J, Cohen M, et al. Defeet of hepatoeyte growth factor seeretion by fibroblasts in idiopathic pulmonary fibro- sis. Am J Respir Crit Care Med, 2003, 168:1156-1161.
  • 6Gossart S, Cambun C, Orfila C, et al. Reactive oxygen intermediates as regulators of TNF-alpha production in rat lung inflammation in- duced by silica. J Immunol, 1996, 156: 1540-1548.
  • 7王欣燕,吴晓梅,康小文,黄昆,李波,陈复辉,白露.骨髓间质干细胞移植对博莱霉素诱导大鼠肺纤维化的治疗作用[J].中国呼吸与危重监护杂志,2009,8(1):57-62. 被引量：14

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二级引证文献8

北京师范大学学报（自然科学版）
2008年 第3期

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