增殖细胞核抗原在大肠腺瘤和腺癌中的研究

Study of Proliferating Cell Nuclear Antigen in Colorectal Adenoma and Adenocarcinoma

用鼠抗PCNA单克隆抗体PC10研究大肠肿瘤60例，分成5组：腺癌伴轻度不典型增生10例，腺瘤伴中度不典型增生13例，腺瘤伴重度不典型增生12例，高分化腺癌14例和中分化腺癌11例。实验结果：①PCNA增殖活性在大肠腺癌组显著高于腺瘤组。②PCNA增殖活性在各腺癌组间，以及伴不同程度不典型增生腺瘤组间均无显著差异。③腺癌病例的结肠粘膜增殖类型均明显达到隐窝表面，而腺瘤病例多数达到表面，但有37％病例增殖活性细胞仅限于下1／5～4／5范围。表明PCNA活性测定可用于大肠肿瘤良恶性鉴别及肿瘤发生学的研究。

Summary in the study we ed mouse anti-PCNA monoclotal antibody: PC10 was used forinvestigating PCNA activity in 60 cases with colorectal adenocarcinoma or adenoma. The mainfindings were as follows: 1. The PCNA proiferative activity was higher in the cases with colorectaladenocarcinoma than with adenoma(P<0. 01). 2. There was no significant difference in the PCNAproliferative activity (P> 0. 05) between the group of well diffentiated adencoarcinoma the ofmoderate differentiated adenocarcinoma, and so did between groupo of adenoma with mild,middle andsevere dysplasia. 3. In the adenocarcinoma the proliferative patterm increased obviously to thesuperfictal portions of crypt ;in adenoma 37% cases were from compartment 1 to IV and not reachedthe surface of ctrpt. In conclsion the study demonstrates that the detection of PCNA activity is auseful approach for differentiating the benign and malignant tumor in largs bowel and founded abasisfor further studying carcinogenesis.