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全反式维甲酸对人类骨髓间充质干细胞CD106、CD54表达影响的体外研究

Effects of all trans retinoic acid on CD106 and CD54 expression in human bone marrow mesenchymal stem cells in vitro
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摘要 目的探讨体外全反式维甲酸（ATRA）对人类骨髓间充质干细胞（BM—MSC）CD106和CD54表达的影响。方法用流式细胞术检测0．01、0．1、1．0、10．0μmol／LATRA处理后BM—MSC细胞表面CD106和CD54的表达，采用MTT法测量细胞生长曲线。结果0．01、0．1、1．0、10．0μmol／L的ATRA能增加BM—MSC细胞表面CD54、CD106的表达。结论ATRA对BM—MSC细胞CD54、CD106有上调的作用。 Objective To explore the effect of all trans retinoic acid （ATRA） on the expression of CD106 and CD54 in human bone mesenchymal stem cells and cell growth curve in vitro. Methods The expression of CD54 and CD106 of human BM-MSC was detected by flow cytometry, and cell growth curve of human BM-MSC was tested by MTT assay after coculturing with 0.01, 0.1, 1.0 and 10.0 μmol/1 ATRA, respectively. Results The expression of CD54 and CD106 is increased by 0.01, 0.1, 1.0 and 10.0 μmol/l ATRA in vitro. Conclusion The expression of CD54 and CD106 of human BM-MSC was up-regulated by ATRA.
作者 唐君玲 张力 刘霆 李晓明
机构地区 泸州医学院附属医院血液内科 泸州医学院附属医院口腔外科 四川大学华西医院血液内科
出处 《白血病．淋巴瘤》 CAS 2008年第3期161-163,共3页 Journal of Leukemia & Lymphoma
关键词 维甲酸 间质干细胞 细胞黏附分子 Tretinoin Mesenchymal stem cell Cell adhesion molecule
分类号 R3 [医药卫生—基础医学]
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参考文献7

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二级参考文献11

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白血病．淋巴瘤
2008年 第3期

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