胶体金免疫层析法在布鲁氏菌病不同流行地区的现场应用

On-the-spot application of colloidal gold-immunochromatographic assay in various epidemic areas of brucellosis

目的验证胶体金免疫层析法(GICA)在布鲁氏菌病不同流行区现场应用的价值。方法选择布鲁氏菌病不同流行区人群,采用GICA、斑点金免疫渗滤法(DIGFA)和试管凝集试验(SAT)平行检测布鲁氏菌病人群抗体。结果GICA、DIGFA和SAT检测重流行区、监测地区和非疫区人群抗体阳性率,3种方法之间差异无统计学意义(P>0.05);GICA和SAT平行检测布鲁氏菌病重疫区人群抗体结果,二者阳性符合率为94.2%。GICA和SAT平行检测布鲁氏菌病非疫区人群抗体结果,二者阴性符合率为99.6%。GICA检测不同疫区之间人群抗体阳性率差异有统计学意义(P<0.01)。结论GICA不仅可及时快速发现患者,而且可监测疫情,反映流行程度,在布鲁氏菌病不同流行区具有较好的推广应用前景,对巩固布鲁氏菌病防治成果具有重要意义。

Objective The study was conducted evaluate the on-the-spot performance of Colloidal Gold-Immunochromatographic Assay （GICA） in various epidemic areas of brucellosis. Methods Parallel comparison across GICA, dot immunogold filtration assay （DIGFA） and serum agglutination test （SAT） was made in different epidemic areas of brucellosis to evaluate the performance of the three methods in detecting human anti-brucella antibodies, Results No statistical difference was found in the positive rates of human anti-brucella antibodies in epidemic areas, surveillance areas and non- epidemic areas detected by the three methods （P〉0.05）. The positive coincidence was 94.2% between GICA and SAT when they were applied in epidemic areas, and the negative coincidence was 99.6% when applied in non-epidemic areas. The positive rates of human anti-brucella antibodies identified by GICA were statistically distinguishable across different epidemic areas （P〈0,01）, Conclusion Highly sensitive to potential patients, GICA can be used as a surveillance indicator of the extent to which the epidemic has prevailed. Its wide application is to be expected in different epidemic areas of burcellosis, which will contribute to the prevention and management of this disease,