外源性三磷酸腺苷-氯化镁预处理对大鼠供肝热缺血损伤的保护作用

Protective effect of exogenous ATP-MgCi2 preconditioning on warm ischemia injury of rat liver graft

目的观察三磷酸腺苷-氯化镁（ATP—MgCl2）预处理对无心跳大鼠供肝热缺血损伤的保护作用。方法根据ATP—MgCl2预处理与否及供肝获取前经历的供体心脏停搏时间（即热缺血时间）30min或45min，将实验动物分为4组，即非预处理的30min（N-30min）组和45min（N-45min）组，以及ATP-MgCl2预处理的30min（tN-30min）组和45min（tN-45min）组行原位肝移植，观察存活状况，取材行光学显微镜及电子显微镜检查，移植术后1、3、7d采集血样检测肝功能。结果N-30mitt组和N-45min组的1周存活率分别为50．0％和16．7％，而tN-30min组和tN-45min组的1周存活率分别为83．3％和66．7％，预处理组移植肝脏的病理及肝功能明显好于非预处理组。结论ATP—MgCl2预处理能够减轻供肝的热缺血损伤，改善肝功能，减轻病理损害，提高大鼠肝移植的存活率。

Objective To explore the peotective effect and mechanism of ATP-MgC12 precondi- tioning on warm ischemia injury of the liver from non-heart-beating donor （NHBD） in rats. Methods According to pretreatment with ATP-MgC12 or not and the non heart beating time of 30 or 45 min before donor liver was harvested, the rats were divided into 4 groups： the group of non heart beating for 30 rain （N-30 min） ,the group of non heart beating for 45 min （ N-45 rain） ,the group of treatment and non heart beating for 30 min （tN-30 min） ,and the group of treatment and non heart beating for 45 min （tN-45 min）. Liver transplantation was performed. The survival and the biopsy of hepatic graft for histological and subcellular structures were observed under a microscope and electronic microscope. Plasma ALT, LDH and HA at 1 st, 3rd and 7th day（s） after transplantation were assayed. Results One-week survival rate in N-30 min and N-45 min groups was 50% and 16.7% respectively,and that in tN-30 min and tN-45 min group was 83. 3% and 66.7% respectively. Conclusion ATP-MgC12 preconditioning can relief warm ischemia injury of rat liver graft from NHBD,improve the liver graft function, lessen pathological changes, and enhance survival rate of rats bearing liver transplantation.