Cuff套管技术在同系大鼠肺移植中的应用

Application of cuff technique in orthotopic lung transplantation of rats

目的建立同系大鼠原位肺移植模型，评价Cuff套管技术应用效果。方法采取两名手术者同时、分别行供受体手术的方法，行左侧同系原位大鼠肺移植22例，肺动脉、肺静脉、主支气管吻合均采用非缝合的Cuff套管技术，评价其可行性和可靠性。结果移植手术成功率90．9％（20／22），移植物缺血时间、热缺血时间和总的手术时间分别为（42．5±2．3）min，（17．1±3．6）min和（68．7±5．1）min。同系大鼠移植术后3周以内（12／22）通气和血流灌注良好，手术后6个月（3／22）、9个月（3／22）、12个月后（2／22）移植肺显示通气不良但吻合口开放。结论大鼠原位肺移植模型采用非缝合的Cuff套管技术行支气管和血管吻合简便、快速和可靠，支气管吻合后能够保持较长时间的管腔开放。

Objective To estimate whether cuff technique can well be employed for rat left lung transplantation. Methods Orthotopic left lung transplantation was performed on 22 rats with nonsuture cuff technique by two persons who operated on the donors and recipients simultaneously and separately. The feasibility and reliability of this technique was determined. Results The success rate of transplant operation was 90. 0% （20/22）. Graft ischemic time, warm ischemic time and total operative time was （42.5 ± 2.3） min, （17.1 ±3.6） min and （68.7 ±5.1） min respectively. Isografts at 3rd week （14/22） looked well peffused and ventilated, and over 6 months （3/22） ,9 months （3/22） and 12 months （2/22） all demonstrated ventilation but looked hypoperfused. Coneluslon The nonsuture external cuff technique is a reliable, simple and fast method for performing orthotopic left lung transplantation in the rat. In contrast to earlier reports patency of the bronchus was found even at long term.