羊栖菜中清除自由基活性物质的研究

Studies on the free radical scavenging activity of bioactive substances from Sargassum fusiforme

目的通过不同溶剂对羊栖菜的有效成分进行提取和体外活性检测,以期筛选出具有一定开发潜力的自由基清除剂。方法采用DPPH(二苯代苦味酰自由基)体系、超氧阴离子体系对不同提取物进行清除自由基活性测定,并与海带、紫菜的提取物及抗坏血酸(VitC)和茶多酚作活性对照。结果不同溶剂对羊栖菜的提取率不同,各种提取物之间的清除自由基活性存在一定差异。结论0.5%Na2CO3溶液提取率最高,且提取物清除自由基的能力最强,以DPPH体系作为检测系统时测量结果较为稳定,效果也较明显。

Objective Bioactive components were extracted from Sargassum fusiforme by using different solvents and their activities were detected in vitro in order to screen potential free radical scavenger. Methods Using the assay system of DPPH, superoxide radical, and in comparison with vitamin C , tea polyphenols and a series of extracts of sea tangle,laver. Resuits Different solvents had different extraction rates, and extracts by different solvents had different free radical scavenging activities. Conclusion 0.5%Na2CO3 solution had the highest extraction rate and extracts by this solution had the best scavenging ability as well. At the same time, the results obtained by DPPH detection method were more stable than those by the others.