绵萆薢药材质量控制的实验研究

The Experiment Study for Quality Control of Diascorea septemloba Thunb

以薯蓣皂苷元为对照品，采用三氯甲烷-乙酸乙酯（10：1）为展开剂，对绵萆薢药材进行薄层色谱鉴别；采用HypersilODS2C18柱（5μm，4．6mm×250mm），乙腈-水（90：10）为流动相，203nm为检测波长，测定绵萆薢药材中薯蓣皂苷元的含量。绵萆薢药材薄层色谱鉴别中，薯蓣皂苷元与其他成分分离良好，能较好的鉴别出薯蓣皂苷元；HPLC定量分析中，薯蓣皂苷元进样量2．16～19．44μg范围内呈良好的线性关系，平均回收率为100．92％，RSD为1．30％。本方法简便，结果准确，重现性好，可以用于绵萆薢药材的质量控制。

Diascorea septemloba Thunb were identified by TLC respectively; using diosgenin as reference substances,and （10 ： 1） as mobile phases. Diosgenin contents were determined by HPLC using the ODS reversed-phase column of Hypersil C18 （5μm, 4.6mm × 250mm）, the mobile phase was a combination of acetonitrile and water（90 ： 10）,and the eluate was monitored with UV absorption at 203nm. Diosgenin was well-separated by TLC. The diosgenin contents in D. septenloba Thunb by HPLC,in the linear range of 2.16 to 19. 44μg and the recoveries of 100. 92% （RSD = 1.30 %）. The established methods are simple, feasible and reproducible. It is also proposed to be used for the quality control of the D. septemloba Thunb.