槐耳清膏对K562细胞体外作用研究

Effects of extractum trametes robiniophila murr on K562 cells in vitro

目的通过用不同浓度的槐耳清膏作用于体外培养的K562细胞,探讨槐耳清膏对K562细胞的增殖抑制及凋亡诱导作用。方法应用MTT法、细胞形态学观察以及流式细胞术观察槐耳清膏对体外培养的K562细胞的增殖抑制及凋亡诱导作用。结果MTT法结果显示:在终浓度分别为0.5、1、2、4和8mg/ml的槐耳清膏作用于体外培养的K562细胞12、24、36、48、72h后,槐耳清膏抑制细胞增殖作用随着浓度增加及培养时间延长而逐渐增强,与对照组相比差异统计学意义(P<0.01)。流式细胞技术显示:不同浓度的槐耳清膏作用于K562细胞48h后,随着浓度的增加,凋亡细胞百分比逐渐增加,与对照组相比差异具有统计学意义(P<0.01)。结论槐耳清膏在体外对K562细胞有抑制增殖和诱导凋亡的作用。

Objective To investigate the effects of extractum trametes robiniphila murr on the proliferation and apoptosis of K562 cells, cells were treated with different concentrations of extractum trametes robiniphila murr. Methods K562 cells were cultured in vitro, and logarithmic phase cells were used for study. MTT-inhibitory test and cell morphological analysis were used to examine the effccts of extractum trametes robiniphila murr on the proliferation of K562 cells. The effects of extractum trametes robiniphila murr on apoptosis of K562 cells were examined by flow cytomery. Results MTT results showed that the inhibition ratio of K562 cells proliferation increased and had a dose-and time-dependent manner after cells were treated with 0.5, 1, 2, 4 and 8 mg/ml concentration of extractum trametes robiniphila murr for 12, 24, 36, 48 and 72 h. Compared with the control group, the difference had statistical significance （P〈0.01）. IC50 of this polusacchaide in 12, 24, 36, 48 and 72 h was separately 7.86, 4.64, 3.06, 1.92 and 1.84 mg/ml. Flow cytomery method results indicated that the apoptosis ratio of K562 cells fbr 48 h increased with the increasing ofconcentration of extractum trametes robiniphila murr, and the difierence had statistical significance（P〈0.01） compared with the control group. Extractum tramctcs robiniphila murr could induce cell apoptosis in a dose-dependent manner. Conclusion Extractum trametes robiniphila murr could inhibit proliferation and induce apoptosis of K562 cells in vitro.