诺氟沙星荧光光度法测定微量Al(Ⅲ)

Determination of micro amounts of Aluminum(Ⅲ) by fluorescence ehancement method with norfloxacin capsules

铝对荧光试剂诺氟沙星有强的荧光增强作用，据此建立了测定微量铝的荧光分析方法。选择最大激发与发射波长分别为278nm和441nm，在pH4．10的HAc-NaAc介质中，诺氟沙星的荧光强度变化与Al（Ⅲ）浓度呈良好的线性关系，线性范围为7．00×10^-6～1．00×10^-3mol／L，检出限为2．00×10^-8mol／L，常见的共存离子不干扰测定。本方法已用于实际样品中Al（Ⅲ）的测定。

A new and sensitive fluorimetric method for the determination of micro amounts of Al（ Ⅲ ） was reported. It was based on the fluorescence ehancement effect of Al （ Ⅲ ） on norfloxacin capsule solution. In HAc-NaAc of pH 4.10, the maximum excitation and emission wavelengths of norfloxacin capsules were found to be 278 nm and 441 nm, respectively. There is a good linear relationship for the concentration ranged from 7.00 × 10^-6 mol/L to 1.00 × 10^-3 mol/L, with the detection limit of 2.00 × 10^-8 mol/L. Some common anions and cations do not interfere. It has been applied to the direct determination of Al（ Ⅲ ） in the samples, and the satisfactory results have been obtained.