铊(Ⅲ)-赤藓红体系分光光度法测定万古霉素

Spectrophotometric Determination of Vancomycin Using Thallium(Ⅲ)-Erythrosin System

在pH为5．3～5．5的乙酸一乙酸钠缓冲液中，铊（Ⅲ）能氧化赤藓红而使之褪色，其最大褪色波长位于526nm处，而加入万古霉素能抑制该褪色反应的进行．基于此反应，发展了一种简便，灵敏和快速的测定万古霉素的分光光度法．该方法线性范围0.2～2．0μg／mL，摩尔吸光系数7．02×10^5L／（mol·cm），检出限66．0ng／mL．文中考察了方法适宜的反应条件和影响因素，检验了共存物质的影响，表明方法具有良好的选择性，可应用于市售万古霉素针剂中万古霉素含量测定．

In pH 5.3--5.5 HAc-NaAc buffer solution, thallium（Ⅲ） could oxide erythrosin; which resulted in the hypochromicity and the maximum fading wavelength was located at 526 nm. Adding vancomycin into the system ,coul;t inhibit this fading reaction. Based on the above phenomena, a simple, sensitive and fast spectrophotometric method has been developed for the determination of vancomycin. The linear range of this method was 0, 242. 0μg/mL and the molar absorpitivity was 7.02× 10^5 L/（mol · cm） and the detection limit was 66.0 ng/mL. The optimum conditions for the reaction and the mpact factor were investigated . The interference of foreign substances was examined and it showed this method obtained good selectivity. The proposed method .ean. be applied to the determination of vancomycin in the commercial iniection.