摘要
目的观察眼镜蛇毒对大鼠脑干神经元型一氧化氮合酶(nNOS)表达的影响,探讨其对前庭神经核的作用。方法采用免疫组织化学方法,观察nNOS阳性细胞在眼镜蛇毒组、生理盐水组、正常对照组大鼠前庭神经核中的表达,并对其作比较。结果蛇毒组前庭神经核nNOS表达与正常对照组、生理盐水组比较有明显下调(P<0.01);蛇毒组前庭神经核nNOS阳性细胞灰度值与正常对照组、生理盐水组比较明显增高(P<0.01),蛇毒对前庭神经核nNOS阳性细胞形态影响不明显。结论眼镜蛇毒对前庭神经核nNOS表达呈下调作用。
Objective To investigate the effects of Naja naja atra venom on the expression of nNOS in brain stem of rat, specially in vestibular nerve nuclei of rat. Methods The methods of immunohistochemistry technique were used to observe the different expression of nNOS positive cells in vestibular nerve nuclei of Naja naja atra venom group, saline group and control group. Results The number of nNOS positive of Naja naja atra venom group were lower markedly than that of saline and control groups （P〈0. 01）. The celluar average optic density value of Naja naja atra venom group was higher than NO-Naja naja atra venom group（P〈 0. 01）. There were no obviously morphological change between them. Conclusion Naja naja atra venom could decrease the expression of nNOS in vestibular nerve nuclei of rats.
出处
《蛇志》
2008年第2期88-90,共3页
Journal of Snake
基金
皖南医学院中青年科研基金资助课题(项目编号:WK200206A)
