从海洋生物鲎血中提取超氧化物歧化酶及其抗菌作用的研究

Study on extracting superoxide dismutase from side product of Tachypleus Amebocyte Lysate and the antibacterial effect of the superoxide dismutase

目的综合利用鲎试剂生产的废料血浆提取超氧化物歧化酶(Superoxide dismutase,SOD),探讨鲎血SOD对耐甲氧西林金色葡萄球菌(MRSA)、对甲氧西林敏感金色葡萄球菌(MSSA)、野生型绿脓杆菌(PAO1)、大肠杆菌(E.coli)的抑菌作用。方法采用离心分离、丙酮沉淀、热变性去杂蛋白的方法从生产鲎试剂废料血浆中提取SOD,然后把SOD加入菌液培养,24h后观察结果。结果SOD对耐甲氧西林金色葡萄球菌、对甲氧西林敏感金色葡萄球菌、野生型绿脓杆菌、大肠杆菌的最低抑菌浓度分别为20.02,40.05,20.02,20.02μg/ml。结论本方法可成功地从生产鲎试剂废料血浆中提取得到SOD粗品,实验表明鲎血SOD具有抗菌作用。

Objective To study the antibacterial effect of the superoxide dismutase （SOD） extracted from side product of Tachypleus Amebocyte Lysate（TAL） on MRSA, MSSA, PAO1 and E. coll. Methods The SOD was extracted from the side product of TAL by separating with a centrifuge, precipitating in acetone, and then heating denaturalization to remove miscellaneous proteins. MRSA, MSSA, PAO1 and E. coli were respectively exposed to the SOD of different concentrations for 24 hours, and the antibacterial effect of the SOD was able to be observed immediately. Results The minimal inhibiting concentration of the SOD to MRSA, MSSA, PAO1 and E. coli was 20.02 μg/ml, 40. 05 μg/ml, 20. 02 μg/ml, 20. 02 μg/ml respectively. Conclusion The rough product of SOD can be extracted from side product of TAL successfully, and shows antibacterial effect.