杜氏利什曼原虫遗传转化中潮霉素及嘌呤霉素适宜浓度的筛选

STUDY ON THE OPTIMAL MASS CONCENTRATION OF HYGROMYCIN AND PUROMYCIN IN LEISHMANIA DONOVANI TRANSFORMATION

通过基因打靶构建基因缺失株是利什曼原虫分子水平研究的有效方法,而筛选特定的转化体是研究成功的第1步。筛选转化体常用抗生素筛选法。本文通过观察潮霉素和嘌呤霉素不同质量浓度对杜氏利什曼原虫四川分离株的生长抑制情况,确定了在抗性基因转化的阳性克隆筛选中,潮霉素及嘌呤霉素的适宜筛选浓度分别为32和10μg/mL。本实验的结果为下一步筛选杜氏利什曼原虫基因缺失株提供了可靠的实验依据。

Generation of gene deletion mutant by gene targeting is an effective method on Leishmania research on molecular level and screen of specific transformant is an important step. The popular method for transformant selection is to add antibiotics. The selection effects on Leishmania donovani with different mass concentration of hygromycin and puromycin were determinded. The results showed that the optimal mass concentration of hygromycin and puromycin was 32 μg/mL and 10 μg/mL respectively, which could provide a credible experimental data for selection of gene deletion mutant of Leishmania donovani.