膀胱癌患者外周血来源树突状细胞诱导培养及表型分析

Study on the induction culture and phenotypes of dendritic cells originating from peripheral blood of bladder cancer patients

目的:探讨膀胱癌患者外周血来源树突状细胞(Dendritic cell,DC)诱导培养方法,观察其形态学特点及分子表型变化。方法:从膀胱癌患者外周血中分离出单个核细胞,在GM-CSF、IL-4的诱导下培养扩增DC,光学显微镜下观察培养过程中的形态学变化,流式细胞术检测其分子表型变化。结果:诱导培养7天即可获取大量成熟DC,细胞表面出现典型树枝状突起,免疫组织化学检测证实为CD1a+;流式细胞仪表型分析显示DC特异性标志CD1a的阳性表达率为57.4%,特异性成熟标志CD83的阳性表达率为72.1%,HLA-DR为94.6%。结论:膀胱癌患者外周血单个核细胞在体外可定向诱导培养为成熟的DC,该研究结果为进一步膀胱癌临床生物学治疗奠定了基础。

Objective： To culture and proliferate dendritic cells from the peripheral blood of patients with bladder cancer; to observe the morphologic and phenotypie characteristics of these cells.Methods： The mononuelear cells is olated from peripheral blood were cultured with the supplement of GM - CSF and IL - 4 to generate dendritic cells. The morphologic characteristics of those cell were observed with light microscopes and the phenotypic figures were analysed with FACS. Results： Mature dendritic cells were obtained seven days after induction culture. These cells showed numerous typical dendritic protuberances on their surface. The specific antigen expressed as follows： CDla：57.4% ,CD83：72.1%, HLA - DR：94.6%. Conclusion：The mature dendritic cells can be obtained by culturing the peripheral monoeytes blood of baldder cancer patients, which lays the further foundation for clinical biotherapy of baldder tumor.