缺血性卒中急性期患者红细胞调控白细胞介素-18新的实验体系研究

Studies on a New Experimental System of Erythrocyte Regulating IL-18 in Patients with Acute Ischemic Stroke

目的:采用血液免疫反应路线图实验体系探讨缺血性卒中急性期患者红细胞调控白细胞分泌IL-18的能力。方法:将缺血性卒中急性期患者组分为2组即大、中小梗死组。将癌细胞(S180)悬液0.2mL(10×106/mL)或生理盐水0.2mL加至枸橼酸抗凝全血细胞悬液(或白细胞悬液)0.2mL和血浆0.3mL中,37℃水浴1h,酶联免疫法测定反应液中IL-18含量。结果:大梗死组IL-18的含量明显高于中小梗死组和对照组(P<0.01)。中小梗死组无抗原激活组(0.32±0.34)高于抗原激活组(-0.41±0.74)和对照组无抗原激活组(-0.20±0.50)红细胞对白细胞分泌IL-18的调控率(P<0.05)。结论:动脉粥样硬化阶段就已经存在红细胞免疫功能的下降,梗塞时更为明显。红细胞对白细胞分泌IL-18具有吸附下调作用,疾病严重时处于失调控阶段。

Objective To approach the capacity of erythrocyte regulating IL-18 in patients with acute ischemic stroke （AIS） by a new experimental system of hemaimmune. Methods Patients with AIS were divided into two groups of the large, the midst and small by the volume of infarction and made group comparison. 0. 2 mL suspension of cancer cells （S180：10 ×106/ml） or NS were added into 0.2 mL anticoagulant suspension of whole blood cells or leukocytes and 0.3 mL plasma, then incubated for 1 h at 37℃. The content of IL-18 was determined by ELISA. Results The content of IL-18 in the. large group was much higher than those of the others. The regulative capacity of erythtocyte to IL-18 with cancer cells was lower than without in the midst and small group, and also was lower than that of the control group with cancer cells. Gonclusion When in the period of atherosclerotic, the immunity erythrocyte had been declined. And it declined more when in AIS. It could absorb IL-18, while it was in imbalance when the disease was serious.