基于16SrDNA序列分析及特异PCR鉴定DVS中未知乳杆菌

Identification of An Unknown Lactobacillus from DVS by Sequence Analysis of 16SrDNA and Special PCR

目的:探讨EZAL-MY96直投式酸奶发酵剂(DVS)中未知乳杆菌的分子生物学鉴定方法。方法:采用显微观察初步鉴定,利用16SrDNA序列分析方法进一步鉴定,利用NCBI中BLAST数据库对测序结果进行比对,构建系统发育树进行分析,并对菌种进行特异PCR分析。结果:最终确定该未知乳杆菌为德氏乳杆菌保加利亚亚种(Lactobacillus delbrueckiisubsp.Bulgaricus)。结论:基于16SrDNA序列分析及特异PCR结合的方法可以快速、准确地的鉴定出未知乳杆菌。

Objective： A molecular biology method was investigated for identifying an unknown Lactobacillus isolated in lactic acid bacteria from EZAL - MY96. Method： An unknown Lactobacillus was initially identified through micro - observation. Then, identification of the strain was performed using the 16SrDNA sequence analysis. Furthermore, the sequence analysis by using the NCBI BLAST database was described and analyzed using phylogenetic tree. And special PCR for strain was performed. Result：The strain was finally identified as Lactobacillus delbrueckii subsp. Bulgaricus. Conclusion： An unknown Lactobacillus from DVS was identified quickly and exactly by sequence analysis of 16SrDNA and special