电气石对绿豆培养细胞生长的影响

Tourmaline Ceramic Balls Stimulate Growth of Mung Bean Cultural Cells

目的:研究电气石对植物细胞生长的影响。方法:绿豆(Vigna radata Wilczek)胚乳细胞悬浮培养中添加电气石,测定电气石对细胞数目和生物量增长的作用及其浓度效应。结果:电气石能够促进绿豆悬浮细胞生长。在30mL的悬浮液中分别加入3、6、9和12g电气石,3g电气石处理对细胞生长的促进作用不显著;12g电气石处理表现出过量反应。6g和9g电气石处理对细胞生长的促进作用较理想,处理10d后细胞数目分别比对照增加33%和38%;6g电气石处理12d后细胞的生物量为对照的1.8倍。电气石处理对细胞大小无显著影响。结论:电气石通过促进细胞增殖而显著促进绿豆细胞生长和生物量的增加。

Objective： To explorer possible function of tourmaline on cell growth of mung bean （ Vigna radata Wilczek） suspension cultural cells. Method： Tourmaline ceramic balls were added directly into cultural solution of mung bean suspension cells. Cell numbers and biomass of the suspension cultures were detected during a 12d culture. Result： Application of 3, 6, 9 or 12g of tourmaline ceramic balls in a 30ml suspension culture, 3g of tourmaline showed limited growth promotion, while 12g had an overdose reaction. 6 and 9g of tourmaline promoted cell growth significandy, which increased cell numbers by 33% and 38%, respectively. In the treatment with 6g of tourmaline ceramic balls, the biomass of culture cells was increased 80% after 12d culture, cell size was not affected. Conclusion： Results indicate that tourmaline ceramic balls stimulate significantly growth of mung bean suspension cells through cell proliferation.