黄芪甲甙对小鼠柯萨奇B3病毒性心肌炎的抗氧化作用

Protective Effect of Astragaloside on Viral Myocarditis in Mice with CVB3

目的观察黄芪甲甙对小鼠柯萨奇B3病毒性心肌炎的抗氧化作用。方法Balb/c小鼠50只随机分5组(每组10只),空白对照组腹腔无菌注射不含病毒的Eagle’s培养基0.1 mL,在腹腔注射病毒培养基30 min后,以生理盐水0.1 mL灌胃,共7d;病毒性心肌炎对照组小鼠每只腹腔注射0.1 mL内含1×102TCID50柯萨奇B3病毒的Eagle’s培养基,在腹腔注射含病毒的Eagle’s培养基30 min后,以生理盐水0.1 mL灌胃,共7 d;黄芪甲甙低、中、高剂量干预组在腹腔注射病毒30 min后,用黄芪甲甙剂量分别为0.07、0.2、0.6 g/(kg.d)0.1 mL灌胃,共7 d。观察小鼠生存数;心肌病变积分;心肌谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶活力及逆转录聚合酶链反应检测铜锌超氧化物歧化酶-mRNA水平表达。结果(1)与空白对照组比较,病毒性心肌炎对照组小鼠的生存数明显下降,心肌病变积分明显升高(3.23±0.83,P<0.01);与病毒性心肌炎对照组比较,黄芪甲甙高剂量干预组可明显提高小鼠的生存数(P<0.01),心肌病变积分明显下降(1.86±0.59比3.23±0.83,P<0.01);(2)与病毒性心肌炎对照组比较,黄芪甲甙高剂量干预组心肌组织谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶活力呈增高趋势,心肌组织谷胱甘肽过氧化物酶(39.73±8.02比9.99±2.71,P<0.05)、过氧化氢酶(6.35±2.33比1.36±0.87,P<0.05)、超氧化物歧化酶(65.71±4.93比40.15±6.03,P<0.01)活力明显增强,铜—锌超氧化物歧化酶-mRNA水平表达明显增高(0.43±0.11比0.32±0.01,P<0.01)。但黄芪甲甙低、中剂量干预组,虽然也在一定程度使心肌组织谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶活力及逆转录聚合酶链反应检测铜—锌超氧化物歧化酶-mRNA表达水平增加,有一定的量效关系,但与病毒性心肌炎对照组比较无统计学意义(P>0.05)。结论黄芪甲甙通过增加心肌抗氧化酶活力,对小鼠柯萨奇B3病毒性心肌炎具有明显的保护作用。

Aim To study protective effect and the anti-oxidize mechanism of Astragaloside on viral myoearditis in mice with CVB3. Methods Fifty Balb/c mice were randomized into five groups （n= 10）： normal control group, given 0.1 mL of EMEM by intraperitoneal injection, were teated with saline 0.1 mL with garage after 30 minities of injection for 1 week; viral myocardifis Control group, inoculated intraperitoneaUy with 0.1 mL of 1 × 10^2 TCID50 LWB3 diluted in Eagle＇ s minimal essential medium （EMEM） solution were given saline 0.1 mL with gavage after 30 minities of injection for 1 week; Astragaloside low-dose intervention group , middle-dose intervention group and high-dose intervention group, inoculated intraperitoneally with 0.1 mL of 1 × 10^2 TCID50 CVB3 diluted in Eagle＇s minimal essential medium （EMEM） were treated with 1%, 3% and 9% astragaloside [0.07, 0.2 and 0.6 g/（kg·d）, respectively] 0.1 mL solution after 30 minetes of injection, respectively with gavage for 1 week. Survival number, score of pathological changes,GSH-PX, CAT, SOD and CuZn-SOD-mRNA of myocardium were detected. Results The survival number was significantly improved in Balb/c mice treated with high dose astragaloside group than that in viral myocarditis control group （ P 〈 0.01 ）. Heart function was better in high-dose intervention group than in the viral myocarditis control group, the activity of GSH-PX, CAT, SOD and CuZnSOD -mRNA levels was enhanced and heart function was improved in group high dose astragaloside group than in viral myocarditis control group （ P 〈 0. 01 ）. Conclusion The results showed that astragaloside can provide protection against viral myocarditis. This protective effect of astragaloside may be related to the maintenance of the antioxidant status of the heart in improving myocardial antioxidant enzymes activity .