两种兔肝VX2模型建立方法的比较及对介入治疗的意义

Comparison of establishing rabbit model bearing VX2 tumor in two methods and its meaning for interventional therapy

目的:比较两种兔肝VX2模型建立方法的差异,并探讨其对肝癌经肝动脉化疗栓塞(TACE)和经皮消融治疗实验的意义。方法:40只家兔分成A、B两组,每组20只,A组行开腹后肝穿刺瘤组织块悬液注入法,B组行开腹后肝组织内包埋法。2周后用彩色多普勒超声观察肝脏及腹腔情况,后处死家兔观察肝脏肿瘤生长情况及腹腔转移情况,并取肿瘤组织行HE染色确定是否为肿瘤组织。统计学处理采用χ2检验。结果:A、B两组家兔术中出血情况及接种用时情况无明显差异;接种2周后行彩色多普勒超声检查A组发现肝脏占位性病变11例,B组发现19例,肝脏病变发现率A组明显低于B组(P<0.05);处死家兔后,家兔仅肝脏种植部位有肿瘤灶生长A组10例,B组16例,B组明显高于A组(P<0.05)。肝脏成功种植A组17例,B组19例,无统计学差异(P>0.05)。结论:开腹后肝组织内瘤组织块包埋与瘤组织块悬液注入法相比,接种成功率无明显差异,但前者肝内外转移灶少于后者,较适合TACE和经皮超声消融治疗的实验研究。

Objective： To compare the difference of two methods of establishing rabbit model bearing VX2 liver tumor and explore its meaning for trans-catheter arterial chemoembolization and percutaneous ablation therapy. Method： Forty Japanese white rabbits were divided into two groups randomly, 20 in each group. VX2 tumor pieces mixed with sodium chloride were injected into the liver by laparotomic route in group A and VX2 tumor pieces were implanted under the hepatic capsule in group B. The observation included： （1）the liver and abdominal cavity by color Doppler ultrasound after two weeks. （2）The growth of tumor in liver and metastasis in the abdominal cavity after killing the rabbits. （3）Identify the tumor tissue by HE staining. Differences of the two groups were statistically assessed with χ^2 tests. Result： The mean period of surgery and bleeding were not significant in the two groups. The rabbits with liver neoplasms in group A was 11 cases and group B was 19 cases seen by color Doppler ultrasound. The discovery rate of liver mass in group B was higher than in group A （P〈0.05）. Having liver neoplasms only in the transplanted site of group A was 10 cases and group B was 16 cases. Group B was higher than group A （P〈0.05）. The cases of successful transplanted carcinoma in group A was 17 and in group B was 19. There were no statistically difference between the two groups（P〉0.05）. Conclusion： The successful rate of transplanted carcinoma had no significant difference between embedding and injecting method through abdominal incision, but ectopia implantation in the former is few, it suits empirical study of trans-catheter arterial chemoembolization and percutaneous ablation therapy.