西藏小型猪H-FABP基因的PCR-RFLP研究

Polymorphism of Heart Fatty Acid-Binding Protein Gene in Tibet Minipig Populations Detected by PCR-RFLP

目的研究西藏小型猪心脏脂肪酸结合蛋白(H-FABP)基因5′-上游区和第二内含子内的遗传变异。方法应用PCR-RFLP技术测定30头西藏小型猪H-FABP的基因型。结果(1)在5′-上游区的HinfI-RFLP位点上,西藏小型猪表现出多态性,等位基因h的频率为0.80;(2)在在第二内含子内的HaeⅢ-RFLP位点上,西藏小型猪均为DD纯合子;(3)在第二内含子内的HinfI*-RFLP位点上,除一头猪表现为bb基因型外,其余猪都表现为BB基因型,等位基因B的频率为0.97;(4)除HaeⅢ-RFLP位点外,在其余位点上西藏小型猪均处于Hardy-Weinberg不平衡状态。(5)在HinfI-RFLP中,西藏小型猪表现为中度多态性(0.25<PIC<0.5),而在其他位点的RFLP中表现为低度多态(PIC<0.25)。结论可以利用西藏小型猪H-FABP基因5′-上游区的多态遗传标记来分析其与肌内脂肪的关系。

Objective To study the genetic variation in 5＇-upstream and the second intron of heart fatty acid-binding protein （H-FABP） gene in Tibet minipigs. Methods PCR-restriction fragment length polymorphism （PCR-RFLP） assay was used to measure the H-FABP genotype in 30 Tibet minipigs. Result （ 1 ） The Tibet minipigs had polymorphism at the Hinf I-RFLP site of the 5＇-upstream region, and the frequency of h allele in Tibet minipigs was 0.80. （2） The Tibet minipigs presented only DD genotype at the Hae Ⅲ-RFLP site; （3） At the Hinf I＊-RFLP site of the second intron, all the Tibet minipigs presented BB genotype except only one minipig presented bb genotype, and the frequency of B allele was 0.97; （4） Other than at the Hae Ⅲ-RFLP site, all the Tibet minipigs populations were not in Hardy-Weinberg equilibrium. （5） The genetic diversity in Tibet minipigs was at a moderate level （0.25 〈 PIC 〈 0.5） at the HinfI-RFL P site while in other sites, the level was low （ PIC 〈 0.25）. Conclusion Genetic polymorphism in 5＇-upstream region of H-FABP gene in Tibet minipigs can be used as a marker for analysis on its relationship with intramuscular fat.