细胞核结构与发生相关基因在大鼠肝再生中表达模式的分析

EXPRESSION PROFILES OF NUCLEAR STRUCTURE AND BIOGENESIS-RELATED GENES DURING RAT LIVER REGENERATION

目的了解大鼠肝再生中细胞核结构与发生相关基因的表达动态。方法用查阅网站资料和相关论文等方法获得上述基因,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用比较手术和假手术组中基因的有意义表达异同,确定肝再生相关基因。结果初步证实上述基因中406个基因与肝再生相关。部分肝切除（PH）后,肝再生早期（0.5～4h）、前期（6～12h）、中期（12～66h）、后期（72～168h）等4个阶段起始表达的基因数为200、29、179和5;基因的总表达次数为374、290、1 876和603。共上调1 224次,下调496次。肝再生前期和中期核形成与发生相关基因表达增强;中期核被膜、核基质和核质相关基因表达增强;中期和后期核染色体、核仁和核功能蛋白复合体相关基因表达增强。结论细胞核结构与发生相关基因在大鼠肝再生中表达活跃,并与肝再生密切相关。

Objective To study the expression changes and profiles of nuclear structure and biogenesis-related genes in rat liver regeneration （LR）. Methods Genes involved in nuclear structure and biogenesis were obtained by data collection and literature review. The gene expression changes during LR were checked by Rat Genome 230 2.0 array, and LR-related genes were identified by comparing gene expression difference between partial hepatectomy （PH） and sham operation （SO） groups. Results 406 genes were found to be LR-related. The numbers of initially and totally expressed genes occurring in the forepart（0.5-4 hours after PH） of LR, the prophase（6-12 hours after PH） ,the metaphase（12-66 hours after PH）, and the anaphase（72-168 hours after PH） were 200, 29, 179, 5 and 374, 290, 1 876, 603, respectively. The numbers of their up-regulation and down-regulation were 1 224 and 496 times. The elevated transcription levels occur red to the nuclear organization and biogenesis-involved genes in the prophase and the metaphase of LR and to the nuclear membrane, nuclear matrix and nucleoplasm-involved genes in the metaphase; nuclear chromosome, nucleolus and nuclear functional protein complex-involved genes were up-regulated in the metaphase and the anaphase. Conclusion The expression of nuclear structure and biogenesis-related genes was active during LR, and it was tightly related with LR.