转录因子基因在大鼠肝再生中的表达方式和作用分析

EXPRESSION PATTERNS AND ROLES OF TRANSCRIPTION FACTOR GENES IN RAT LIVER REGENERATION

目的探讨转录因子基因在肝再生中的表达变化及作用。方法经查阅网站资料和相关论文获得转录因子基因及其参与的生理活动,用大鼠基因组230 2.0芯片检测转录因子基因在大鼠再生肝中的表达,用手术组和假手术组比较的方法确定肝再生相关基因。结果初步证实上述基因中有320个基因与肝再生相关,涉及细胞代谢、增殖、分化、凋亡等16种生理活动。它们在肝再生中的表达分为41种方式,表明肝再生中细胞生理生化活动具有阶段性、多样性和复杂性。其中,肝再生早期[部分肝切除（PH）后0.5～4h]和前期（PH后6～12h）糖类合成,脂类代谢和炎症反应相关转录因子基因表达增强,中期和后期细胞增殖、生长、分化和凋亡相关转录因子基因表达增强。结论肝再生的生理生化活动受多种转录因子基因调控。其中,e2f1、fos、copeb等转录因子基因发挥关键作用。

Objective To study the expression changes of transcription factor genes and their underlying involvement in rat liver regeneration （LR） at transcription level. Methods The transcription factor genes and their function were collected by referring to the putative literatures and databases. Then, their expression in rat LR were checked by Rat Genome 230 2.0 array. LR-related genes were identified through the comparison of discrepancies in gene expression between partial hepateetomy （PH） and sham-operation （SO） groups. Results 320 genes were determined to be LR-related, which were involved in 16 biological processes, such as cellular metabolism, proliferation, differentiation, apoptosis, etc. Expression profiles of above genes were classified into 41 categories according to their functions and expression trends, implying their diversity and complexity during LR. The expression of transcription factor genes that were involved in carbohydrate biosynthesis, lipid metabolism, and inflammatory response was enhanced in the forepart （0.5-4 hours after PH） and prophase （6-12 hours after FH） of LR. Cellular proliferation, growth, differentiation and apoptosis related transcription factor genes were promoted in the metaphase （ 16-66 hours after FH） and anaphase （72-168 hours after PH）. Conclusion Physiological and biochemical activities in LR were regulated by multiple transcription factor genes, and e2fl, fos, copeb, etc. played key roles in it.