超短波对兔激素性股骨头缺血性坏死骨组织BMP-2mRNA的影响

The effect of ultrashortwave therapy on the expression of BMP-2 in the early stages of steroid-induced avascular necrosis of the femoral head

目的观察超短波对兔激素性股骨头缺血性坏死骨组织中骨形态形成蛋白-2表达的影响。方法30只新西兰大白兔分为正常组（4只）和干预组（26只），干预组采用马血清联合激素复制兔激素性股骨头坏死的模型，经X线验证造模成功的有20只，再随机分为超短波组（10只）和造模组（10只）。造模组不予任何干预；超短波组给予超短波治疗，第一个疗程15d，无热量；第二个疗程15d，微热量，治疗间隔5d。空气栓塞快速处死动物，并取兔双侧股骨头，应用实时荧光定量PCR检测骨组织中BMP-2mRNA的基因表达情况。结果造模结束时，干预组BMP-2的表达为正常组的0．2001倍（P〈0．01），差异有统计学意义；第一疗程结束时，超短波组BMP-2mRNA表达量为造模组的2．738倍，第二疗程结束时，超短波组BMP-2mRNA表达量为造模组的10．1260倍（均P〈0．01），差异具有统计学意义。结论超短波可以提高激素性股骨头坏死骨组织BMP-2mRNA的表达，从而促进缺血性坏死股骨头的修复。

Objective To observe the effect of ultrashortwave therapy on the expression of BMP-2 in steroid-induced avascular necrosis of the femoral head （ SANFH ） . Methods Thirty New-Zealand white rabbits were randomly divided into two groups： a normal group （4 rabbits） and an intervention group （26 rabbits）. An animal model of steroid-induced avascular necrosis of the femoral head was induced through the intravenous injection of horse blood serum and the intraperitoneal injection of methylprednisolone in the intervention group. X-ray examination confirmed the successful induction of SANFH in 20 of the rabbits, who were then subdivided at random into an ultrashortwave therapy group （ 10 rabbits） and a model group （ 10 rabbits）. The rabbits in the ultrashortwave group were treated with ultrashortwave therapy in two 15-day courses, with a 5-day interval between them. Non-thermal ultrashortwave therapy was given in the first course, then mild thermal ultrashortwave therapy was given in the second. Those in the model group were reared without ultrashortwave therapy. All the animals were sacrificed immediately after the end of the last treatment, and their femoral heads were sampled to observe the expression of BMP-2 using real time fluorescent quantitative PCR. Results The expression of BMP-2 mRNA in the intervention group was only one fifth of that observed in the normal group. It was 2.7 to 10 times that observed in the model group. Both differences were significant at the 1% confidence level. Conclusion Ultrashortwave therapy can promote the expression of BMP-2 and improve reactivation in avascular necrosis of the femoral head.