p120蛋白在膀胱非浸润性乳头状尿路上皮癌中表达的研究

Abnormal expression of p120 in non-invasive papillary urothelial carcinoma of human bladder

目的了解p120在膀胱非浸润性乳头状尿路上皮癌(NPUC)以及浸润性尿路上皮癌(IUC)的表达情况。方法使用HE、免疫组化等方法检测48例膀胱癌标本,28例为膀胱NPUC、20例为IUC,10例为正常膀胱黏膜组织p120、E-cad、p63的表达情况,并结合其病理分级及临床资料进行分析。结果10例正常膀胱黏膜上皮细胞膜p120和E-cad均(+),28例NPUC的异常表达率分别为57%(16/28)和36%(10/28),而IUC均出现两蛋白的异常表达。其中两蛋白在低级别和高级别NPUC的表达差异显著(P<0.01和P<0.05),但与肿瘤大小、患者性别及年龄差异不显著。结论p120在膀胱癌表达异常,有可能成为膀胱NPUC新的标记物,对判断肿瘤预后有参考意义。

Objective To investigate the p120 expression in non-invasive papillary urothelial carcinoma （NPUC） and invasive urothelial carcinoma （IUC） in bladder. Methods We used HE and immuno-histochemical technique to detect the immunoreactivity and cellular localization of p120 and E-cadherin in 48 cases of NPUC and IUC as well as 10 normal bladder biopsies, and correlated these results with pathological and clinical parameters. Resets P120 and E-cadherin were expressed in a normal membranous pattern in all normal bladder epithelium specimens. Loss of normal surface p120 and E-cadherin expression was found in all of IUC, the same results was also detected in NPUC with the expression rates of 16/28 （57%） and 10/ 28 （36%）, respectively. Furthermore, there was a significant difference in the membranous expression of p120 and E-cadherin between the low grade NPUC and the high grade NPUC （ P 〈 0.01 and P 〈 0.05）, and no obvious relationship was found among the tumor size, age and sex. Conclusion Aberrant expression of p120 may be a new marker of NPUC in bladder, which plays a role in evaluation of the prognosis.