血管内皮生长因子-C mRNA的表达与食管鳞癌临床病理特征的关系

VEGF-C mRNA expression and its relationship with clinicopathological parameters in esophageal sqamnous cell carcinoma

目的探讨血管内皮生长因子-C（VEGF—C）mRNA的表达与食管鳞癌临床病理特征的关系。方法采用实时荧光定量逆转录多聚酶链反应（QRT—PCR）法，测定59例食管癌和正常黏膜中VEGF—CmRNA的表达。结果食管癌组织中VEGF—CmRNA表达显著高于正常黏膜（6．30和2．81，P=0．02），淋巴结转移者的VEGF—CmRNA表达显著高于无转移者（10．11和4．15，P=0．04），转移淋巴结≥4枚患者的VEGF—C mRNA表达显著高于〈4枚的患者（62，19和6，30，P=0．01），转移淋巴结组≥3组患者的VEGF—C mRNA表达显著高于〈3组的患者（18.98和4.92，P=0．04），Ⅱb＋Ⅲ＋Ⅳ期患者的VEGF-C mRNA表达显著高于Ⅰ＋Ⅱa期患者（9.99和3．80，P=0.03），颈淋巴结转移与区域淋巴结转移患者的VEGF-CmRNA表达无统计学意义。Logistic多元回归分析显示，VEGF—C mRNA高表达是食管癌淋巴结转移的独立危险因素（P=0．01）。单因素和多因素分析均显示，EGFR—C mRNA表达与食管鳞癌患者的预后无关，而淋巴结转移组数是食管癌预后的独立危险因素（P〈0.01）。结论VEGF—C mRNA的表达与食管鳞癌淋巴结转移密切相关，在食管鳞癌的淋巴结转移中起着非常重要的作用。

Objective To detect the expression of VEGF-C mRNA and to investigate its relationship with clinicopathological parameters in esophageal squamous cell carcinoma （ESCC）. Methods Real-time quantitative reverse transcriptase-PCR was used to measure the level of VEGF-C mRNA in the tumor tissue and corresponding normal mucosa in ESCC patients. Results The VEGF-C mRNA expression in tumor tissue was significantly higher than that in the corresponding normal mucosa （6. 30 vs. 2.81, P = 0.02）, and also significantly higher in the patients with lymph node metastasis than that in those without lymph node metastasis （ 10.11 vs. 4.15, P = 0.04）. Among the patients with metastatic lymph nodes, VEGF-C mRNA expression was 62.19 in the patients with ≥4 metastatic lymph nodes versus 6.30 in those with 〈4 （P = 0.01）, and 18.98 in the patients with ≥ 3 metastatic lymph node stations versus 4.92 in those with 〈 3 （P = 0.04）. In terms of stage, VEGF-C mRNA expression was significantly higher in the stage Ⅱ b ＋ Ⅲ ＋ Ⅳ than that in the stage Ⅰ ＋ Ⅱ a （9.99 vs. 3.80, P = 0.03）. Logistic binary regression analysis showed that VEGF-C mRNA was an independent risk factor for lymph node metastasis in ESCC （P = 0.01 ）. In survival analysis, 2-year survival rate was not related with VEGF-C mRNA expression （ P = 0.46）. It was showed by COX regression model that the number of metastatic lymph node stations was the only independent risk factor for survival （P 〈 0.01 ）. Conclusion The expression of VEGF-C mRNA play an important role in lymph node metastasis of human ESCC.