PANDER基因在胃癌中的表达下调

Down-regulation of PANDER mRNA in gastric cancer

目的:研究配对胃癌组织及转移淋巴结组织中中PANDER基因表达,并分析其与胃癌临床病理及预后的相关性.方法:用RT-PCR检测22例配对胃癌及对应切缘正常胃黏膜组织中PANDER mRNA的表达;制备PANDER cRNA探针,并构建胃癌组织芯片,用原位杂交方法检测208例配对的胃癌组织及转移淋巴结中PANDER基因的表达,分析PANDER基因表达与胃癌临床病理及预后之间的相关性.结果:RT-PCR显示16/22例(72.7%)胃癌中PANDER基因表达下降.原位杂交证实从正常胃黏膜至转移淋巴结,PANDER阳性率依次降低(χ2=81.135,P=0.00).PANDER基因在60.9%胃癌组织中表达下降,早期胃癌与进展期胃癌表达下降的比率无差别(χ2=5.362,P=0.147).肿瘤组织PANDER基因表达与浸润深度有关,局限在黏膜和黏膜下层浸润者PANDER表达高于已经浸润至肌层和浆膜层者(Z=-2.52,P=0.012);胃癌组织中PANDER基因表达与胃癌预后无关.结论:胃癌组织中PANDER表达的下降是胃癌早期事件,可能与胃癌的发生、发展相关.

AIM： To investigate the expression of PANDER gene in paired gastric cancer tissues and metastatic lymphatic tissues, and to evaluate its relationship with the clinicopathologic characteristics and disease prognosis. METHODS： Reverse transcription-polymerase chain reaction （RT-PCR） was used to examine the expression of PANDER mRNA in 22 fresh surgical samples of primary gastric cancer tissues and their paired surrounding normal gastric mucosa. A gastric cancer tissue microarray containing 1020 duplicate matched normal mucosa, malignant tissues and metastatic lymphatic tissues from 208 gastric cancer patients was constructed. In situ hybridization analysis was performed on the tissue microarray and the correlation between PANDER mRNA expression and clinicopathologic factors was analyzed. RESULTS： RT-PCR showed that the expression of PANDER mRNA decreased in 72.7% （16/22） of gastric cancer samples. Similar results were obtained by in situ hybridization analysis （60.9%, 112/184）. The expression of PANDER mRNA showed an ordinally decreasing trend in the matched normal mucosa, tumor tissues and metastatic lymphatic tissues （x^2 = 81.135, P = 0.00）. There was no difference between early gastric cancer and progressive gastric cancer （x^2 = 5.362, P = 0.147）. In tumor tissues, lower expression of PANDER mRNA was associated with deeper invasion （Z = -2.52, P = 0.012）. There was no correlation between PANDER mRNA expression and disease prognosis. CONCLUSION： Down-regulation of PANDER gene expression occurs at the early stage of gastric cancer, which may be involved in the genesis and development of gastric cancer.