CpG对HBV感染者B细胞及Th1型细胞因子的活化作用

Immunostimulatory effects of CpG-ODN 2216 on the activation of peripheral blood B lymphocytes and Th1-type cytokines from patients with hepatitis B virus infection

目的:观察CpG-ODN 2216对乙型肝炎病毒(hepatitis Bvirus,HBV)感染者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中B淋巴细胞抗原提呈相关分子CD80、CD86、MHC-Ⅰ和MHC-Ⅱ分子表达的影响,同时观察CpG对Th1型细胞因子分泌的影响.方法:取HBV感染者PBMC与CpG-ODN2216共培养48h,用流式细胞技术检测B淋巴细胞表面CD80、CD86、MHC-Ⅰ和MHC-Ⅱ分子的表达,并与未加CpG组进行比较,同时检测培养液中Th1型细胞因子白介素-12(IL-12)和干扰素-γ(IFN-γ)的水平.结果:经CpG-ODN2216活化刺激后,PBMC中B淋巴细胞抗原提呈相关分子CD80、CD86、MHC-Ⅰ及MHC-Ⅱ分子的表达显著高于对照组(CD80:48.84%±21.29%vs28.57%±18.7%;CD86:50.12%±19.70%vs13.15%±8.81%;MHC-Ⅰ:2108.88±289.04vs1679.22±388.22;MHC-Ⅱ:1602.77±362.61vs941.88±237.35;均P<0.05);并且培养液中IFN-γ及IL-12的水平也显著高于对照组(IFN-γ:61.38±38.81ng/Lvs47.35±38.76ng/L;IL-12:7.80±4.34ng/Lvs5.56±3.56ng/L;均P<0.05).结论:CpG-ODN2216能够提高HBV感染者B淋巴细胞表面抗原提呈相关分子和共刺激分子的表达,同时提高PBMC分泌Th1型细胞因子的能力.

AIM： To determine the effects of immunostimulatory action of CpG-ODN 2216 on the activation of B lymphocytes and Thl-type cytokines derived from peripheral blood mononuclear cells （PBMCs） of patients infected with hepatitis B virus （HBV）. METHODS： PBMCs from donors infected with HBV were cultured in the presence of CpG-ODN 2216 for 48 h; B lymphocytes were analyzed for CD19/CD86, CD19/CD80, major histocompat-ibility complex （MHC） class Ⅱ and class Ⅰ using flow cytometry. The supernatant levels of interleukin-12 （IL-12） and interferon-γ（IFN-γ） were detected by enzyme-linked immunosorbent assay （ELISA）. RESULTS： CpG-ODN 2216 promoted a strong up-regulation of CD86, CD80, MHC-Ⅰ and MHC- Ⅱ on B lymphocytes in HBV-infected patients （CD80： 48.84% ± 21.29% vs 28.57% ± 18.70%; CD86： 50.12% ± 19.70% vs 13.15%± 8.81%; MHC-Ⅰ： 2108.88 ± 289.04 vs 1679.22 ± 388.22; MHC-Ⅱ： 1602.77 ± 362.61 vs 941.88 ± 237.35; all P 〈 0.05）. IL-12 and IFN-γ levels in the slimulated supernatant were increased significantly （IFN-γ： 61.38 ± 38.81 ng/L vs 47.35 ± 38.76 ng/L; IL-12： 7.80 ± 4.34 ng/L vs 5.56 ± 3.56 ng/L; both P 〈 0.05）. CONCLUSION： CpG-ODN 2216 up-regulates the expression of B lymphocyte antigen presenting and costimulatory molecules on the surface of B lymphocytes derived from HBV-infected patients, and promotes the capability of PBMCs in secreting Th1-type cytokines.