hPOT1 RNA干扰对人胃癌BGC823细胞TRF1、TRF2和Tankyrase1表达的影响

Effect of inhibition of hPOT1 by RNA interference on gene expression of TRF1, TRF2 and Tankyrasel in human gastric cancer cell BGC823

目的观察人端粒保护蛋白（hPOT1）RNA干扰对胃癌BGC823细胞端粒重复序列结合因子1（TRF1）、端粒重复序列结合因子2（TRF2）、端锚聚合酶（Tankyrasel）转录水平的影响。方法采用半定量RT—PCR方法检测TRF1、TRF2和Tankyrasel在mRNA表达水平的改变。结果hPOT1RNA干扰抑制细胞中hPOT1表达后，TRF1表达明显上调，TRF2和Tankyrase1明显下调。结论人胃癌BGC823细胞中hPOT1表达下调伴随TRF1表达明显上调、TRF2和Tankyrasel表达明显下调，提示hPOT1和TRF1、TRF2、Tankyrase1三个端粒相关蛋白之间具有较为密切的联系，并且相互制约、相互影响。

Objective To investigate the effect of silence of human protection of telomeres 1 （ hPOT1 ）, which was induced by RNA interference, on expression of telomeric repeat factor 1 （ TRF1 ）, telomeric repeat factor 2 （ TRF2 ） and Tankyrase 1 in human gastric cancer cell BGC823. Methods The expression of TRF1 ,TRF2 and Tankyrasel at mRNA level were determined by semi-quantitatlve RT-PCR. Re- sults Significant increase in expression of TRF1, marked decrease of TRF2 and Tankyrasel at mRNA level were observed in cells of hPOTI siRNA. Conclusion The significant increase in expression of TRF1 and the marked decease in TRF2 and Tnakyrasel at mRNA level after the inhibited expression of hPOT1 in human gastric cancer cell BGC823 indicate that hPOT1 is highly correlated with the expressions of other three telomere-specific binding proteins.