蛋白质组技术在胃癌相关标志物筛查中的应用

Application of technologies of proteome for screening molecular biomarkers associated with gastric cancer

目的初步探讨蛋白质组技术在血清研究中的应用,建立胃癌患者血清和健康者血清双向凝胶电泳图谱;筛查并鉴定胃癌患者血清和健康者血清中的差异表达蛋白质,期望从中发现有意义的胃癌分子标志物。方法采用双向凝胶电泳(2-DE)分离低分化胃癌患者血清和健康者血清总蛋白,银染显色,ImageMaster 2D Elite软件分析,从中选取差异表达蛋白质,通过基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)鉴定差异表达的蛋白质。结果获得分辨率较好的血清双向凝胶电泳图谱;得到4个在胃癌与健康者血清中有显著性差异的蛋白质斑点进行鉴定,结果表明这些特异蛋白分别为Serpin B6(Placental thrombin inhibitor)(Cytoplasmic antiproteinase)(CAP)(Protease inhibitor6)(PI-6),Septin-1(LARP)(Serologically defined breast cancer antigen NY-BR-24),Kallikrein-6 precursor(Protease M)(Neurosin)(Zyme)(SP59),Hemoglobin beta chain。结论在低分化胃癌患者血清与健康者血清中存在差异表达蛋白质,利用蛋白质组学这一高通量的筛查技术可望从中发现与低分化胃癌相关的标志物。

Objective To investigate the application of proteomics in the field of serology, and to screen the differential expression proteins related with gastric carcinoma. Methods Two-dimensional electrophoresis was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining, the differential expression proteins were analyzed using ImageMaster 2D Elite software, and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry （MALDI-TOF-MS） . Results Electrophoresis figures with high reproducibility were obtained. The four high-abundance gastric cancer protein spots were identified and analyzed. The special protein spots were Serpin B6 （Placental thrombin inhibitor） （Cytoplasmic antiproteinase）（CAP）（Protease inhibitor6） （PI-6）, Septin-1（LARP） （Serologically defined breast cancer antigen NY-BR-24）, Kallikrein-6 precursor（Protease M） （Neurosin） （Zyme） （SP59）, Hemoglobin beta chain. Conclusions Protein expression is differenti in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.