大豆抗疫霉根腐病基因SSR标记

Tagging SSR Markers for Resistant Gene to Phytophthora Root Rot in Soybean

选用感病大豆品种合丰25和抗病大豆品种抗线2号作为亲本配制杂交组合,获得F2种子。种植F2代种子获得F2:3家系,作为分子标记分离群体,进行大豆抗疫霉根腐病基因SSR标记。共筛选覆盖大豆全基因组的350对SSR引物,其中52对引物在亲本间具有多态性,在F2群体中通过BSA法筛选出与抗疫霉病基因相关的多态性差异引物1对,为Scaa003,位于大豆公共遗传图谱的J连锁群。同时该引物在其他10个抗病品种及4个感病品种中抗、感病谱带检出率均为100%,具有一定的检测通用性,可以为大豆疫霉根腐菌1号生理小种抗性材料的辅助选择提供理论依据。

Phytophthora root rot is an important soybean disease, which can influence the yield. To map SSR makers link with resistant genes to soybean Phytophthora root rot,Hefeng 25 and Kangxian 2 were crossed and the F2seperation population was obtained. 350 pair of SSR primers were screened and 52 pairs have polymorphism. BSA（ Bulked segregation analysis） method was used to screen the polymorphic and diverge primers,one pair of polymorphic pnmer,Scaa003 ,located on LG J was tagged. The examination rate of Scaa003 between 10 sensitive soybean cuhivars and 4 resistant soybean cultivars reached 100%. Result shows that the primer has the common characteristic for the examination,it can provide the theoretic base for selecting the soybean materials which have the resistance to the soybean Phytophthora sojae Race 1.