大豆M型细胞质雄性不育恢复基因SSR标记初步定位

SSR Marker Location for Fertility Restorer Gene of M-Cytoplasmic Male Sterility in Soybean

鉴于细胞质雄性不育(Cytoplasmic Male Sterility,CMS)在作物杂种优势利用中的良好应用前景及分子标记对恢复系选育的应用价值,试验采用SSR分子标记对大豆CMS恢复系WR016恢复基因进行初步定位。利用197对大豆SSR引物对不育系W931A和恢复系WR016单株进行多态性筛选,52对引物在双亲间表现多态性,多态性频率26.39%,进一步利用这52对引物对10株不育系W931A和恢复系WR016单株、不育基因池、可育基因池和半不育基因池进行分析,表明位于A连锁群上的Satt276表现出良好的多态性。再利用合成的Satt276附近的引物进行分析,表明Satt684和Satt572也有良好的多态性。据此,可将大豆M-CMS恢复系WR016的恢复基因初步定位在A连锁群上。

Based on the advantage of cytoplasmic male sterility（CMS） in the application of crop heterosis and the practical value of molecular markers in crop breeding,this experiment applies SSR molecular markers to basically locate the restorer gene of the M- CMS WR016. 197 pair of SSR primers were used to amplify between sterile line W931A and restorter WR016. Results showed 52 pair of primers have polymorphism and the frequency was 26.39%. The 52 pair of polymorphic primers were used to analyze individul plants of W931 A and WR016, sterile,fertile and semi-sterile bulk. The Satt276 on the linkage group A was demonstrated better polymorphism properties. In addition ,the synthesized primers near Satt276 were apphed, and both Satt684 and Satt572 was demonstrated better polymorphism properties. Hence,the restorer gene of the M- CMS WR016 should be located on the linkage group A of soybean.