生防菌诱导水稻抗病性相关酶活性的变化

Dynamic of resistance-related enzymes in rice induced by antagonistic bacterium

将生防菌bio—2和稻瘟病菌（Magnaporthe grisea）接种于正处于分蘖期的水稻植株上，测定其与植物抗病有重要相关的苯丙氨酸解氨酶（Phenlyalanine ammonia lyase PAL）、过氧化物酶（Peroxidase，POD）和多酚氧化酶（Polyphenoase，PPO）活性的变化．结果显示，接种处理后，POD活性明显高于对照，在接种后第5d。稻瘟病菌、稻瘟病菌＋生防菌bio-2处理组的POD活性达到最大值，但到接种后第8d开始略低于对照；稻瘟病菌＋生防菌bio-2、生防菌bio-2处理组，其叶片内的PAL活性仅在第1—2d内高于对照，其后均低于对照；在稻瘟病菌＋生防菌bio-2、生防菌bio-2处理组，其PPO活性除在第2d高于对照外，其余均低于对照．

Rice plants on tillering stage were inoculated with antagonistic bacterium bio-2 （S） or/and Magnaporthe grisea （C） for analyzing the activities of the indicators： phenlyalanine ammonia lyase （PAL）, peroxidase （POD） and polyphenoase （PPO）, which were closely related with plants resistance to diseases. The results showed that the activity of POD was higher than that of the control, and the peaks of POD activity treated with （C） and （ C ＋ S） appeared on the 5^th d, but lower than that of the control on the 8^th d. The activity of PAL and PPO was lower than that of the control except for treatments with C ＋ S and S, whose activity at 1^st or 2^nd d was higher than that of the control.