丹参酮 Ⅱ A 磺酸钠对红细胞内游离钙浓度的影响

Effects of Sodium Tanshinone ⅡA Sulfonate on Human Intraerythrocytic Free Calcium Concentration

丹参酮ⅡＡ磺酸钠对红细胞内游离钙浓度的影响费丽萍１李进禧１郑道声１张世华１章鲁２国外文献报导红细胞变形与其游离浓度有关［１］。国内学者已通过实验证明了２，３－ＤＰＧ含量与红细胞膜稳定性、形态可塑性、耐久性及硬度有关［２］。为了探讨冠心病患者使用丹参后...

he effects of Sodium tanshinone ⅡA sulfonate(DS201) and Danshensu(DS182) on the intraerythrocytic free Calcium of normal human were studied by means of fluorescent probe(Fura2) half quantitative analysis.After administration of Calcium channel agonist KCl, the free Ca~2+ level of intraerythrocytic obviously elevated(P<0.01),compared with the resting level.After adiministration of DS201(4￣100 μg/ml),DS182(100￣300 μg/ml) and Verapamil(0.1￣10 μmol/L) previously,then KCl,the free Ca2+ level significantly decreased(P<0.01),compared with that of the normal control group.Furthemore,the effects of DS201 and DS182 were dosedependent.There was no significant difference in Ca2+ between DS201 and DS182(P>0.05).Resting free Ca^2+ concentration in erythrocyte was markedly altered by DS201 and Ver,not DS182.The results indicated that free Ca^2+ concentration in erythrocyte through potentialdependent Calcium channel could be blocked by DS201 and DS182.These characteristics of DS201 and DS182 were similar to that of Ver.Infering the effects that the erythrocyte deformability of human was incresed obviously by Danshen is related to DS201 and DS182, which can inhibit Ca^2+ influx of erythrocyte.