摘要
采用束缚水浸法造成大鼠急性胃粘膜损伤模型，观察溃疡指数，运用Westerndotblot法检测大鼠血浆及胃组织中热休克蛋白（HSPs）水平的变化。并对目组织的HSPs作免疫组织化学研究、结果表明：热处理组和健脾益气组的溃疡指数极其显著低于模型组（P＜0．01），但血浆及胃组织中的HSPs显著高于模型组（P＜0．05）．溃疡指数与HSPs表达水平呈明显负相关。提示热处理及健脾益气方处理能诱导大鼠提高HSPs的表达，防止胃粘膜损伤，表明HSPs。可能介导胃粘膜防御。
A rat model of acute injury of gastric mucosa was established- The ulcer index wasobserved. The changes of heat shock proteins (HSPs ) in Plasma and gastric mucosa wasdetermined by using Western dot blot and the HSPs in gastric tissues was immunohistochemicallyexamined. Out results showed that the ulcer indices in heat-treated group and Jianpiyiqi-treatedgroup were significantly lower than that in the medel group(P<0. 01 ), but the HSPs in plasmaand gastric tissues in heat-treated group and Jianpiyiqi-treated group were significantly higher thanthat in model group (P <0.05). The ulcer indices were negatively related with expression level ofHSPs, suggesting that heat treatment and treatment by Jianpiyiqi recipe could induce expression ofHSPs in rats, thereby preventing the gastric mucosa from being injured and that the HSPs mightbe involved in the defending mechanism of gastric mucosa.
基金
国家中医药管理局资助!95B151
关键词
健脾益气方
中药
方剂
热休克蛋白
胃粘膜损伤
Jianpiyiqi recipe
heat shock proteins
acute injury of gastric mucosa
