摘要
1H-1HCOSY和13C-1HHSQC谱对通过Bio-GelP-2柱(2.5cm×125cm)分离制备的纤维三糖、纤维四糖和纤维五糖进行分析。结果表明,在水溶液中纤维低聚糖还原末端C1位α构型与β构型之比(α/β)随聚合度增大而减小,纤维三糖主要是α构型(α/β,0.52),而纤维四糖和纤维五糖主要是β构型(α/β,分别是0.34和0.25)。X射线光电子能谱(XPS)分析表明纤维低聚糖的C1s主要有2种电子结合能,分别为284.8(C1s1,C—O)和286.1eV(C1s2,O—C—O),C1s1与C1s2之比(C1s1/C1s2)随聚合度增大而增大。
^1H-^1H COSY and ^13C-^1H HSQC spectra were used to assign cellotriose, cellotetraose and cellopentaose preparatively separated on Bio-Gel P-2 column （2.5 cm × 125 cm）. The results illustrate thatthe configuration ratios of α-form to β-form （α/β） at C1 of the reducing end decrease with the increase of degree of polymerization （Dp） for cellooligosaccharides in aqueous solution. The configuration for cellotriose is mainly the α-form （ α/β, 0.52）, while for cellotetraose and cellopentaose are mainly the β-form （ α/β, 0.34 and 0.25 respectively）. X-ray photoelectron spectra （XPS） analysis shows that the binding energy of C ls was mainly assigned to 284.8 and 286. 1 eV corresponding to C lsl （C--O） and C ls2 （O--C--O）, respectively. The ratios of C lsl/C ls2 increase with the increase of Dp for cellooligosaccharides.
出处
《林产化学与工业》
EI
CAS
CSCD
2008年第3期61-65,共5页
Chemistry and Industry of Forest Products
基金
高等学校科技创新工程重大项目培育项目(705048)
长江学者和创新团队发展计划(IRT0552)
关键词
纤维低聚糖
分离
核磁共振光谱
X射线光电子能谱
cellooligosaccharides
fractionation
NMR spectroscopy
X-ray photoelectron spectra
