山稔子中脂肪酸的气相色谱-质谱联用分析

GC-MS Determination of Fatty Acids in Fructus rhodomyrti Seeds

乙醚作溶剂,超声波提取,氢氧化钾/甲醇衍生,气-质联用法分析,测定了稔子油脂类中脂肪酸的组成。结果表明:稔子油含有14种脂肪酸,脂肪酸组成中亚油酸GC含量84.99%,其次是饱和脂肪酸,占14.55%,包括4-甲基戊酸、己酸、十四烷酸、十五烷酸、十六烷酸、十七烷酸、十八烷酸、二十烷酸、二十二烷酸。油酸GC含量0.28%,除此之外,还含有少量的共轭亚油酸(CLA)。

The extract was extracted from the seeds of Fructus rhodomytri using ether assisted by supersonic wave. The extract was transesterified in KOH-CH30H and analyzed by GC-MS method equipped with 25 m × 0.22 mm capillary column. The fatty acids in F. rhodomyrti seeds were determined. The results showed that there were 14 fatty acids in which linoleic acid, saturated fatty acids and oleic acid are the chief components and their relative contents were 84.99 % , 14.55 % and 0.28 % , respec- tively. Among saturated fatty acids are 4-methypentanoic acid, hexanoic acid, octanoic acid, tetradecanoic acid, hexadecanoic acid, heptadecanoic acid, n-octadecanoic acid, eicosanoic acid and docosanoic acid. In addition, there was a little amount of conjugated linoleic acid （CLA）.